AI Article Synopsis
- Researchers developed fluorescent flipper probes for imaging membrane tension in living cells, utilizing both twisted and planarized mechanophores.
- The probes can switch between a bright state and various dark states when exposed to certain conditions, allowing for effective tension measurement.
- Using advanced imaging techniques, the flippers provide highly detailed and stable images of cellular membranes, surpassing traditional resolution limits.
Article Abstract
We report the design, synthesis, and evaluation of fluorescent flipper probes for single-molecule super-resolution imaging of membrane tension in living cells. Reversible switching from bright-state ketones to dark-state hydrates, hemiacetals, and hemithioacetals is demonstrated for twisted and planarized mechanophores in solution and membranes. Broadband femtosecond fluorescence up-conversion spectroscopy evinces ultrafast chalcogen-bonding cascade switching in the excited state in solution. According to fluorescence lifetime imaging microscopy, the new flippers image membrane tension in live cells with record red shifts and photostability. Single-molecule localization microscopy with the new tension probes resolves membranes well below the diffraction limit.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/jacs.0c04942 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!