To explore the bioaerosol composition characteristics and sources in urban areas and mountains, three sample sites were selected in urban (urban, suburban) and southern mountainous areas in Xi'an, and air, soil, and leaf samples were simultaneously collected. The fungi and bacteria community structures at different sampling sites were analyzed through a high-throughput sequencing method, and their spatio-temporal variations were also examined. Moreover, a source track technique was used to identify the source of microorganisms in the air. The results showed that the fungi and bacteria at different sampling sites were significantly different, indicating that the geographical location has a significant influence on the community structure of microorganisms in the air. In winter, more potential fungal pathogens and bacterial pathogens were detected in urban areas with high relative abundance and diversity. In addition, the results showed that the main local source of fungi and bacteria was the leaf surface, of which the contribution rate of bacteria in the air fine particles was up to (55±30)%. This study can provide a scientific basis for understanding the characteristics of microbial pollution in the atmosphere, and for air environment quality evaluation and disease prevention in China.
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http://dx.doi.org/10.13227/j.hjkx.201911010 | DOI Listing |
FEBS Lett
December 2024
Chemical Resource Development Research Unit, RIKEN Center for Sustainable Resource Science, Wako, Japan.
FOXO3a is a transcription factor involved in cell growth inhibition and apoptosis. FOXO3a is localized in the cytoplasm in cancer cells, and its nuclear translocation by small molecules is expected to prevent cancer cell growth. In this study, we screened a fungal broth library in HeLa cells using fluorescently labeled FOXO3a and an AI-based imaging system.
View Article and Find Full Text PDFYing Yong Sheng Tai Xue Bao
October 2024
Liaoning Ocean and Fisheries Science Research Institute/Key Laboratory of Protection and Utilization of Aquatic Germplasm Resource, Ministry of Agriculture and Rural Affairs/Key Laboratory of Molecular Biology for Marine Fishery, Dalian 116023, Liaoning, China.
We investigated food composition and feeding selectivity of jellyfish () from the coastal aquaculture ponds in Liaodong Bay by DNA metabarcoding technology. The DNA from environmental water samples and stomach contents of were extracted and sequenced by high-throughput sequencing with 18S rDNA V4 region and mitochondrial cytochrome c oxidase subunit I (COI) as metabarcoding markers. Based on 18S rDNA metabarcoding, we detected 27 phyla in the stomach contents of , in which Mollusc was the dominant phylum followed by Arthropod, and 34 phyla in the environmental water samples, in which Pyrrophyta was the dominant phylum followed by Ciliophora and Ascomycota.
View Article and Find Full Text PDFYing Yong Sheng Tai Xue Bao
October 2024
College of Natural Resources and Environment, Northwest A&F University/Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Ministry of Agriculture and Rural Affairs, Yangling 712100, Shaanxi, China.
Inoculating zinc solubilizing microorganisms (ZSMs) is considered as a promising strategy for increasing Zn phytoavailability in soils with low Zn availability. In present study, we screened six strains of ZSMs from rhizosphere of green manure crop, including three strains of fungi, , and three strains of bacteria, . We conducted a pot experiment of Bok choy inoculated with different ZSMs to analyze the Zn content in shoots and roots, and compared the Zn solubilizing effect of ZSMs.
View Article and Find Full Text PDFYing Yong Sheng Tai Xue Bao
October 2024
College of Forestry, Agricultural University of Hebei, Baoding 071000, Hebei, China.
We elucidated the changes of soil microbial biomass and community structure in soil profiles under four typical land use types (farmland, grassland, secondary forest and plantation)and across five soil layers (0-10, 10-20, 20-30, 30-40, 40-50 cm) in the northern mountainous region of Hebei Province. We measured soil microbial biomass by phospholipid fatty acid (PLFA) method, and investigated the effects of land use and soil depth on soil microbial biomass and community structure by variance analysis, correlation analysis and redundancy analysis. The results showed that soil water content, bulk density, and organic carbon content of farmland differed significantly from other land use types.
View Article and Find Full Text PDFMycoses
January 2025
Department of Dermatology and Venereology, Peking University First Hospital, Beijing, China.
Objectives: Tinea capitis remains a common fungal infection in children worldwide. Species identification is critical for determining the source of infection and reducing transmission. In conventional methods, macro- and microscopic analysis is time-consuming and results in slow fungal growth or low specificity.
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