Evaluation of biphenyl- and polychlorinated-biphenyl (PCB) degrading sp. MAPN-1 on growth of by pot study.

This study focused on isolation of bacteria with biphenyl/polychlorinated biphenyl (PCB) degrading ability from the rhizosphere of (mulberry plant). Repetitive enrichment of rhizospheric soil samples with biphenyl resulted in the isolation of sp. MAPN-1, identified by 16S rRNA gene sequence analysis. The bacterium showed growth on five different aromatic compounds (naphthalene, salicylic acid, benzoic acid, dibenzofuran and anthracene). Benzoic acid was detected as the major metabolite during biphenyl degradation using high-performance thin-layer chromatography (HPTLC) with R 0.42 at 254 nm. Further GC-MS/MS study showed 95% and 15% degradation of biphenyl and dichlorobiphenyl, respectively. A pot study was conducted to evaluate the effect of presence of biphenyl on and the role of biphenyl degrader sp. MAPN-1 in relation to phytoremediation. twigs in biphenyl spiked soil (100 mg/kg and 300 mg/kg) inoculated with sp. MAPN-1 showed growth, whereas, growth of plants (control) was adversely affected in biphenyl-spiked uninoculated soil. It is the first report of isolation of sp. MAPN-1 from the rhizosphere of , its capability to degrade biphenyl, thereby showing a positive effect on the plant growth grown in biphenyl spiked soil.