AI Article Synopsis
- * In 2017, 296 blood samples from asymptomatic individuals in Jalalabad were analyzed using various methods, including microscopy and advanced PCR techniques to assess malaria prevalence.
- * Results showed a higher prevalence of asymptomatic malaria (7.78%) when using advanced molecular methods compared to traditional microscopy (1.7%), indicating a significant number of asymptomatic carriers that could perpetuate the disease.
Article Abstract
Background: Malaria is threatening more than half of Afghanistan population. Asymptomatic malaria is notable problem against malaria controlling strategies. In this study we evaluated the asymptomatic malaria status in Nangarhar Province, Afghanistan in 2017.
Methods: Overall, 296 finger blood samples were taken on DNA Banking Cards and microscopic slides from asymptomatic individuals in Jalalabad city. We used a novel post real time PCR high resolution melting analysis beside microscopy and semi-nested multiplex PCR to evaluate status of asymptomatic malaria in this city.
Results: The prevalence of asymptomatic malaria in Jalalabad city was determined 1.7% (5/296), 7.43% (22/296) and 7.78% (26/296) by microscopy, Seminested multiplex PCR and qRT-PCR-HRM, respectively. Out of 26 positive cases were detected by qRT-PCR-HRM, 21, 1 and 4 cases were detected , and mixed infection of and , respectively.
Conclusion: Our data indicating on existence of significant number of asymptomatic reservoirs that assists in prolonged endemicity of the disease. On the other hand, the molecular methods are better alternatives for microscopy especially for monitoring of asymptomatic cases of malaria.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7311817 | PMC |
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