Breeding of high protein Chlorella sorokiniana using protoplast fusion.

To improve Chlorella's economic viability as a natural bait in aquaculture, protoplast fusion technology was used for two Chlorella mutants, H10 and Z13, selected by UV and chemical mutagenesis. Chlorella sorokiniana protoplast was prepared using the enzyme method, and then the optimal enzyme combination of 4% cellulase and 2% driselase was screened out. Z13 and H10 protoplast preparation rates reached 34.72% and 31.11%, respectively. Nine fusions with higher growth rates were selected to assess their biomass, total and soluble proteins contents. Dry cell weight, total protein, and soluble protein of fusion R7 were 0.92 g.L, 67.16%, and 0.59 mg.g, respectively. The biomass was 1.59, 1.43 times that of H10 and Z13; total and soluble proteins increased by 8.89%, 10.25% and 50.12%, 74.62% respectively, compared with the original algae. These results have implications for breeding excellent strains, and for large-scale and optimal application of Chlorella in aquaculture.