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http://dx.doi.org/10.1016/j.jinf.2020.06.055 | DOI Listing |
Food Environ Virol
January 2025
School of Environmental and Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2UW, UK.
Capsid Integrity qPCR (CI-qPCR) assays offer a promising alternative to cell culture-based infectivity assays for assessing pathogenic human virus viability in wastewater. This study compared three CI-qPCR methods: two novel (Crosslinker, TruTiter) and one established (PMAxx dye). These methods were evaluated on heat-inactivated and non-heat-inactivated 'live' viruses spiked into phosphate-buffered saline (PBS) and wastewater, as well as on viruses naturally present in wastewater samples.
View Article and Find Full Text PDFPublic Health Nurs
January 2025
Korea Armed Forces Nursing Academy, Daejeon, Republic of Korea.
Introduction: Disaster nursing is a complex, dynamic, and resource-limited working environment, like military operations. As the COVID-19 outbreak has shown, not only hospitals, but all communities need nurses who can systematically respond to disasters. It is important for nurses to possess the necessary readiness to respond to disasters with confidence and autonomy.
View Article and Find Full Text PDFPediatr Pulmonol
January 2025
The Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Background: Respiratory syncytial virus (RSV) infection in the first year of life has been linked with an increased risk for asthma. Some propose that RSV-induced inflammation leads to lasting airway changes, while others contend that RSV bronchiolitis is a marker for underlying predisposition. Social distancing adopted during the COVID-19 pandemic has led to a dramatic reduction in RSV activity, providing an unexpected opportunity to investigate this debate.
View Article and Find Full Text PDFJ Clin Lab Anal
January 2025
Department of Clinical Biochemistry, Thomayer University Hospital, Prague, Czech Republic.
Background: The longitudinal study was conducted over the initial 2 years of the COVID-19 pandemic, spanning from June 2020 to December 2022, in healthcare workers (HCWs) of the Thomayer University Hospital. A total of 3892 blood samples were collected and analyzed for total nucleocapsid (N) antibodies. The aim of the study was to evaluate the dynamics of N antibodies, their relationship to the PCR test, spike (S) antibodies, interferon-gamma, and prediction of reinfection with SARS-CoV-2.
View Article and Find Full Text PDFEmerg Microbes Infect
January 2025
Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun, Jilin Province 130021, China.
To unravel distinct pattern of metagenomic surveillance and respiratory microbiota between () P1-1 and P1-2 and to explore the impact of the COVID-19 pandemic on epidemiological features, we conducted a multicenter retrospective study which spanned 90,886 pneumonia patients, among which 3,164 cases were identified. Our findings revealed a concurrent outbreak of , with the positivity rate rising sharply to 9.62% from July 2023, compared to the 0.
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