Objectives: This study profiled circulating and hippocampal microRNAs (miRNAs) to identify alterations associated with the risk of epileptogenesis in a mouse temporal lobe epilepsy model.
Methods: Next-generation sequencing was performed to examine the changes in miRNA expression 24 h after pilocarpine-induced status epilepticus (SE) in C57BL/6NCrl mice using both blood and hippocampus samples. Differentially expressed miRNAs were identified from SE animals and matched controls that failed to develop SE after receiving equal doses of pilocarpine (NS animals). Blood and brain miRNA profiles were then compared to identify circulating miRNA alterations reflecting the changes in the brain.
Results: We identified 3 miRNAs that were significantly up-regulated and 4 miRNAs that were significantly down-regulated in the blood of SE animals compared with NS animals. When hippocampal miRNAs of SE animals and NS animals were compared, 5 miRNAs were up-regulated and 4 were down-regulated. Of these, miR-434-3p and miR-133a-3p were observed to have greatest changes in both blood and brain of SE animals.
Significance: This study extends current knowledge of changes in miRNAs associated with epileptogenesis by profiling miRNAs in SE and NS animals in an experimental temporal lobe epilepsy model. The study was designed to allow non-specific changes due to the activation of muscarinic cholinergic receptors in peripheral organs by pilocarpine to be ruled out. Significantly altered circulating miRNAs that reflect changes in the brain during epileptogenesis after SE have the potential to be developed as prognostic biomarkers for epileptogenesis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.eplepsyres.2020.106400 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Retinal Müller Cell-Released Exosomal MiR-92a-3p Delivers Interleukin-17A Signal by Targeting Notch-1 to Promote Diabetic Retinopathy.
Invest Ophthalmol Vis Sci
January 2025
Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Purpose: Inflammatory processes have been involved in diabetic retinopathy (DR). Interleukin (IL)-17A, a pro-inflammatory cytokine, is associated with DR occurrence and development. However, mechanisms underlying the IL-17A impact on DR need further investigations.
View Article and Find Full Text PDFmiR-1224 Controls Mammal Cerebral Cortex Development by Targeting the 3'-UTR of the Dlx1 mRNA.
J Cell Physiol
January 2025
Department of Anesthesiology, The First Affiliated Hospital of Soochow University, Suzhou, China.
Neural precursor cells (NPCs) are a group of cells with self-renewal and multi-differentiation potential. MicroRNAs are required for neurogenesis in the central nervous system (CNS). Recent reports suggest that miR-1224 is important in human CNS diseases.
View Article and Find Full Text PDFUpregulation of LncRNAs G2E3-AS1 and BACE1-AS as Prognostic Biomarkers in Metastatic Colorectal Cancer.
Biomarkers
January 2025
Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Background: Despite the current diagnostic techniques and therapeutic methods for colorectal cancer (CRC), patients are often diagnosed at advanced stages of colorectal cancer with poor prognosis and distant metastasis. Recently, numerous investigations have highlighted the crucial role of lncRNAs in cancer development, progression, invasion, and metastasis. This study investigated less well-characterized genes in the colorectal cancer metastasis process using bioinformatics analysis and their confirmation by experimental methods.
View Article and Find Full Text PDFPolyphenol oxidase cross-linking enhances whey protein-induced systemic food allergy by regulating miRNA in CD4 T cells.
Food Funct
January 2025
State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China.
Whey protein (WP) contains two major allergenic proteins, α-lactalbumin and β-lactoglobulin, which significantly impact its incorporation and application in food products. Current research primarily focuses on the dynamic changes in allergenicity during the processing of individual protein components. To simulate realistic conditions in food processing, this study aims to investigate the effect of polyphenol oxidase cross-linking on the allergenicity of complex protein matrices.
View Article and Find Full Text PDFOne-pot isothermal CRISPR/Dx system for specific and sensitive detection of microRNA.
Anal Methods
January 2025
Department of Colorectal Surgery, College of Clinical Medicine for Oncology, Fujian Medical University, Fuzhou, Fujian, China.
MicroRNA (miRNA) is a promising biomarker for the early diagnosis of pancreatic cancer. To enable sensitive and reliable miRNA detection, we have developed a one-pot isothermal CRISPR/Dx detection system by combining rolling circle amplification (RCA) and CRISPR/Cas12a. RCA and CRISPR/Cas12a reactions are carried out in a single closed tube, bypassing the transferring step.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!