sp. nov., an actinobacterium isolated from freshwater sediment.

A novel, facultatively anaerobic actinobacterium, designated strain CBA3103, was isolated from sediment of the Geum River in South Korea. Phylogenetic analysis indicated that strain CBA3103 is most closely related to LZ-22 (98.47 % 16S rRNA gene sequence similarity). The genome of strain CBA3103 was 3 649 865 bp with a DNA G+C content of 69.6 mol%. The average nucleotide identity value between strain CBA3103 and LZ-22 was 79.22 %. Cells of strain CBA3103 were Gram-positive, rod-shaped, 0.6-0.9 µm wide and 1.4-2.4 µm long. Growth occurred at 15-40 °C (optimum, 35 °C), at pH 6.0-7.0 (optimum, pH 7.0) and with 0-2 % NaCl (w/v) (optimum, 0-1 %, w/v). The major cellular fatty acids in strain CBA3103 were anteiso-C, anteiso-C A and iso-C. The major respiratory quinone was menaquinone-9(H). The polar lipids of strain CBA3103 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, five unidentified glycolipids and three unidentified phospholipids. Based on the genotypic, phenotypic and chemotaxonomic analyses, strain CBA3103 represents a novel species of the genus , for which the name sp. nov. (type strain CBA3103=KACC 21446=DSM 110288) is proposed.