AI Article Synopsis
- The study investigates the relationship between microRNA-7 (miR-7-5p) and fibroblast growth factor receptor 4 (FGFR4) in the context of liver fibrosis, as miR-7-5p acts as a tumor suppressor and is involved in liver health.
- Research methods included predicting miR-7-5p's target (FGFR4), analyzing their expressions in liver tissues, and conducting various assays to examine cell proliferation and activation.
- Findings show that miR-7-5p targets and downregulates FGFR4, leading to increased proliferation and activation of hepatic stellate cells (HSCs), which are essential in liver fibrosis progression.
Article Abstract
Aims: Fibroblast growth factor receptor 4 (FGFR4) is a key mediator that protects the liver from chronic injury. MicroRNA-7 (miR-7) is a tumor suppressor and associated with lipid homeostasis in the liver. This study was designed to examine the role of the miR-7-5p/FGFR4 axis in liver fibrogenesis.
Methods: TargetScan was employed to predict microRNAs that targeted FGFR4 on the 3'-untranslated region (3'-UTR). miR-7-5p and FGFR4 expression in pathological liver tissues and LX-2 cells was determined using qRT-PCR and an immunoblotting assay. A dual-luciferase assay was conducted to validate the target prediction. A Cell Counting Lit-8 assay was performed to assess the proliferation ability of LX-2 cells. Hydroxyproline content in LX-2 cells was measured using a hydroxyproline assay. The expression of hepatic stellate cell (HSC) activation markers was examined using qRT-PCR and an immunoblotting assay.
Results: FGFR4 was a putative target of miR-7-5p. In LX-2 cells, miR-7-5p targeted FGFR4 by binding to 3'-UTR. FGFR4 was downregulated, but miR-7-5p was markedly enhanced in the liver samples as the degree of liver fibrosis rose. miR-7-5p was negatively associated with FGFR4 expression in liver tissues. The miR-7-5p inhibitor blocked the lipopolysaccharide-induced proliferation and activation of LX-2 cells, and FGFR4 overexpression inhibited LX-2 cell proliferation and activation triggered by miR-7-5p.
Conclusion: miR-7-5p promotes HSC proliferation and activation by downregulating FGFR4.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7303738 | PMC |
| http://dx.doi.org/10.1155/2020/5346573 | DOI Listing |
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