Induces Gene Expression in Human Acute Leukemia Jurkat T-Cells.

Objective: T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive disease resulting from the accumulation of genetic changes that affect the development of T-cells. The precise role of lymphoid enhancer-binding factor 1 () in T-ALL has been controversial since both overexpression and inactivating mutations have been reported to date. Here, we investigate the potential gene targets of in the Jurkat human T-cell leukemia cell line.

Materials And Methods: We used small interfering RNA (siRNA) technology to knock down in Jurkat cells and then compared the gene expression levels in the knockdown cells with non-targeting siRNA-transfected and non-transfected cells by employing microarray analysis.

Results: We identified , a tumor suppressor gene, as the most significantly downregulated gene in knockdown cells, and we further confirmed its downregulation by real-time quantitative polymerase chain reaction (qRT-PCR) in mRNA and at protein level by western blotting.

Conclusion: Our results revealed that is positively regulated by in Jurkat cells, which indicates the capability of as a tumor suppressor and, together with previous reports, suggests that exhibits a regulatory role in T-ALL via not only its oncogenic targets but also tumor suppressor genes.