Relationship between enzyme concentration and Michaelis constant in enzyme assays.

The upper bound of enzyme concentration for accurately estimating the parameters in Michaelis-Menten (MM) equation is not completely determined and still under discussion, even though many researchers have investigated the equation's validity for a long time. In the paper, we broadly investigated the correlation between the system of ordinary differential equations for monosubstrate irreversible enzyme reaction (HMM-system) and its derivative MM equation focusing on the relationship between initial enzyme concentration [E] and Michaelis constant K by numerical simulation. According to the results, the initial reaction velocity v is still a function of initial substrate concentration [S] at [E]

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http://dx.doi.org/10.1016/j.biochi.2020.06.002	DOI Listing