AI Article Synopsis
- The study focuses on the late stages of human adenovirus type 5 (HAdV5) infection, specifically its nuclear remodeling in host cells.
- Researchers created virus mutants with fluorescently tagged proteins to observe their behavior during virion production.
- They discovered a specialized area in the nucleus, called the 'late virion accumulation compartment' (LVAC), where viral capsids and DNA are tightly packed and rigid, limiting the movement of larger molecules.
Article Abstract
The human adenovirus type 5 (HAdV5) causes disease of the upper and lower respiratory tract. The early steps of HAdV5 entry up to genome replication in the host nucleus have been extensively studied. However, late stages of infection remain poorly understood. Here, we set out to elucidate the spatiotemporal orchestration of late adenovirus nuclear remodeling in living cells. We generated virus mutants expressing fluorescently tagged protein IX (pIX) and protein V (pV), a capsid and viral genome associated protein, respectively. We found that during progeny virion production both proteins localize to a membrane-less, nuclear compartment, which is highly impermeable such that in immunofluorescence microscopy antibodies can hardly penetrate it. We termed this compartment 'late virion accumulation compartment' (LVAC). Correlation between light- and electron microscopy revealed that the LVAC contains paracrystalline arrays of viral capsids that arrange tightly packed within a honeycomb-like organization of viral DNA. Live-cell microscopy as well as FRAP measurements showed that the LVAC is rigid and restricts diffusion of larger molecules, indicating that capsids are trapped inside.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343190 | PMC |
| http://dx.doi.org/10.1371/journal.ppat.1008588 | DOI Listing |
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