Reactive oxygen species (ROS)-induced oxidative stress has been associated with diseases such as amyotrophic lateral sclerosis, stroke, and cancer. While the effect of ROS on mitochondria and endoplasmic reticulum (ER) has been well documented, its consequence on the Golgi apparatus is less well understood. In this study, we characterized the Golgi structure and function in HeLa cells after exposure to hydrogen peroxide (HO), a reagent commonly used to introduce ROS to cells. Treatment of cells with 1 mM HO for 10 min resulted in the degradation of Arl1 and dissociation of GRIP domain-containing proteins Golgin-97 and Golgin-245 from the -Golgi. This effect could be rescued by treatment of cells with a ROS scavenger -acetyl cysteine or protease inhibitors. Structurally, HO treatment reduced the number of cisternal membranes per Golgi stack, suggesting a loss of -Golgi cisternae. Functionally, HO treatment inhibited both anterograde and retrograde protein transport, consistent with the loss of membrane tethers on the -Golgi cisternae. This study revealed membrane tethers at the -Golgi as novel specific targets of ROS in cells.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7525819 | PMC |
http://dx.doi.org/10.1091/mbc.E20-01-0063 | DOI Listing |
Plants (Basel)
December 2024
Faculty of Agriculture, Dalhousie University, Bible Hill, NS B2N 5E3, Canada.
Drought is an abiotic disturbance that reduces photosynthesis, plant growth, and crop yield. Ascorbic acid (AsA) was utilized as a seed preconditioning agent to assist broccoli ( var. ) in resisting drought.
View Article and Find Full Text PDFPlants (Basel)
December 2024
Institute of Basic Biological Problems of the Russian Academy of Sciences, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", 142290 Pushchino, Russia.
The redox state of the plastoquinone (PQ) pool in thylakoids plays an important role in the regulation of chloroplast metabolism. In the light, the PQ pool is mostly reduced, followed by oxidation after light cessation. It has been believed for a long time that dark oxidation depends on oxygen, although the precise mechanisms of the process are still unknown and debated.
View Article and Find Full Text PDFPlants (Basel)
December 2024
Department of Soil Science, School of Agricultural Science, Federal University of Lavras (UFLA), Lavras 37200-900, MG, Brazil.
Selenium (Se) is an essential element for humans. However, much of the world's human population is deficient in this element, which has become a public health problem. This study aimed to evaluate whether applying severe water stress to wheat plants ( L.
View Article and Find Full Text PDFToxics
November 2024
Guangzhou Vocational College of Technology & Business, Guangzhou 511442, China.
Geogenic arsenic (As) contamination in groundwater poses a significant public health risk in many regions worldwide. Previous studies have reported hydrogen peroxide (HO) concentrations ranging from 5.8 to 96 μmol L in rainwater, which may contribute to the oxidation and removal of As.
View Article and Find Full Text PDFMicroorganisms
December 2024
Oral Care Product Development, The Procter & Gamble Company, Cincinnati, OH 45202, USA.
Various ingredients are utilized to inhibit the growth of harmful bacteria associated with cavities, gum disease, and bad breath. However, the precise mechanisms by which these ingredients affect the oral microbiome have not been fully understood at the molecular level. To elucidate the molecular mechanisms, a high-throughput bacterial transcriptomics study was conducted, and the gene expression profiles of six common oral bacteria, including two Gram-positive bacteria (, ) and four Gram-negative bacteria (, , , and ), were analyzed.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!