-Lactoglobulin (-LG) is a member of lipocalin superfamily of transporters for small hydrophobic and hydrophilic molecules. We located the binding sites of citric acid and gallic acid on -lactoglobulin (-LG) in aqueous solution at physiological conditions, using spectroscopic methods, thermodynamic analysis and molecular modeling. Thermodynamic parameters Δ -9.5 to -6.9 (kJ mol), Δ 23.9 to 13.6 (J molK) and Δ -14.5 to -13.6 (kJ mol) showed that acid binds protein via ionic contacts with gallic acid forming stronger protein conjugates consistent with theoretical modeling. Different amino acids are involved in gallic acid and citric acid complexation. Protein conformation was altered with reduction of β-sheet from 58% (free protein) to 46-43% and a major increase in α-helix from 11% (free protein) to 29-23% and random coil structure in the acid-protein, indicating a partial protein destabilization. Communicated by Ramaswamy H. Sarma.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/07391102.2020.1784290 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Combined transcriptomic and metabolomic analyses reveal the pharmacognostic mechanism of the metabolism of flavonoids in different parts of Polygonum capitatum.
Plant Genome
March 2025
Department of Fundamental Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
The plant Polygonum capitatum (P. capitatum) contains a variety of flavonoids that are distributed differently among different parts. Nevertheless, differentially expressed genes (DEGs) associated with this heterogeneous distribution have not been identified.
View Article and Find Full Text PDF[Effects of Xihuang Pills on angiogenesis, invasion, and metastasis of p rostate cancer based on FAK/Src/ERK pathway].
Zhongguo Zhong Yao Za Zhi
December 2024
Hunan Provincial Key Laboratory of Traditional Chinese Medicine Prescription and Transformation, Hunan University of Chinese Medicine Changsha 410208, China Key Laboratory of Tumor Prevention Mechanism of Traditional Chinese Medicine,Hunan University of Chinese Medicine Changsha 410208, China Key Laboratory of Traditional Chinese Medicine Tumour in Hunan Universities, Hunan University of Chinese Medicine Changsha 410208, China College of Integrative Medicine, Hunan University of Chinese Medicine Changsha 410208, China.
Based on the focal adhesion kinase(FAK)/steroid receptor coactivator(Src)/extracellular regulated protein kinase(ERK) pathway, this study explored the effects of Xihuang Pills on angiogenesis, invasion, and metastasis in prostate cancer. Liquid chromatography-tandem mass spectrometry(LC-MS/MS) was used to analyze and identify the active ingredients of Xihuang Pills. Bioinformatics techniques, including R language and Perl programs, were employed to analyze the interactions between prostate cancer-related targets and the potential targets of Xihuang Pills.
View Article and Find Full Text PDFUltrasmall iron-gallic acid coordination polymer nanoparticles for scavenging ROS and suppressing inflammation in tauopathy-induced Alzheimer's disease.
Biomaterials
December 2024
Department of Nuclear Medicine and PET Center, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China; Institute of Nuclear Medicine and Molecular Imaging of Zhejiang University, Hangzhou, China; Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China; Key Laboratory for Biomedical Engineering of Ministry of Education, Zhejiang University, Hangzhou, China. Electronic address:
Alzheimer's disease (AD) is the most prevalent neurodegenerative disorder globally, with no effective treatment available yet. A crucial pathological hallmark of AD is the accumulation of hyperphosphorylated tau protein, which is deteriorated by reactive oxygen species (ROS) and neuroinflammation in AD progression. Thus, alleviation of ROS and inflammation has become a potential therapeutic strategy in many studies.
View Article and Find Full Text PDFSelective Removal of Highly Toxic Selenite by a Biobased Zirconium-Polyphenolic Supramolecular Gel.
Inorg Chem
January 2025
State Key Laboratory of Tea Biology and Utilization, Agricultural Photocatalysis Laboratory, School of Materials and Chemistry, Anhui Agricultural University, Hefei 230036, China.
The green and facile biobased functional materials have attracted great attention due to the promising potential to deal with the water pollution of toxic selenium ions that act as a serious threat to human health and the ecological environment. The development of cheap and eco-friendly approaches to remove SeO is of great significance for the safety of drinking water. However, there are some disadvantages in most of the employed methods, such as poor removal capability, high cost, and unsustainability.
View Article and Find Full Text PDFPreparation and stability of chebulagic acid and chebulinic acid from Terminalia chebula and their biological activity.
Pak J Pharm Sci
January 2025
College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China/Province Multi-Component Chinese Medicine Engineering Technology Research Center of Liaoning, Dalian, China/Modern Traditional Chinese Medicine Research and Engineering Laboratory of Liaoning, Dalian, China.
Chebulagic acid and chebulinic acid are the two tannin compounds with the highest content in Terminalia chebula, they were separated by ODS column eluted with 20% methanol and 35% methanol, respectively. The compounds were identified by comparing the data of H NMR and C NMR with the literature; HPLC method was used to investigate the stable storage conditions of chebulagic acid and chebulinic acid; lipopolysaccharide (LPS) induced in vivo inflammation model and RAW264.7 macrophage in vitro inflammatory model to evaluate the anti-inflammatory activities of chebulagic acid and chebulinic acid.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!