The COVID-19 pandemic has resulted in an urgent global need for rapid, point-of-care diagnostic testing. Existing methods for nucleic acid amplification testing (NAAT) require an RNA extraction step prior to amplification of the viral RNA. This step necessitates the use of a centralized laboratory or complex and costly proprietary cartridges and equipment, and thereby prevents low-cost, scalable, point-of-care testing. We report the development of a highly sensitive and robust, easy-to-implement, SARS-CoV-2 test that utilizes isothermal amplification and can be run directly on viral transport media following a nasopharyngeal swab without the need for prior RNA extraction. Our assay provides visual results in 30 min with 85% sensitivity, 100% specificity, and a limit of detection (LoD) of 2.5 copies/μl, and can be run using a simple heat block.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7302291 | PMC |
| http://dx.doi.org/10.1101/2020.05.28.20115220 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Aqueous and ethanolic extracts of Moringa oleifera leaves induce selective cytotoxicity in Raji and Jurkat cell lines by activating the P21 pathway independent of P53.
Mol Biol Rep
January 2025
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Background: The traditional use of Moringa oleifera (MO), an essential food source in Africa and Asia, to cure various diseases dates back thousands of years. This study examines the aqueous and ethanolic leaf extracts of MO's in vitro anti-leukemia capabilities.
Methods: After preparing aqueous and ethanolic MO leaf extracts, cells were treated with various concentrations for 48 h.
High-Throughput Dissociation and Orthotopic Implantation of Breast Cancer Patient-Derived Xenografts.
J Vis Exp
December 2024
Division of Exercise Physiology, Department of Health Professions, West Virginia University School of Medicine; Cancer Institute, West Virginia University School of Medicine; 3Department of Microbiology, Immunology, and Cell Biology, West Virginia University School of Medicine;
Patient-derived xenografts (PDXs) provide a clinically relevant method for recapitulating tumor-involved cell types and the tumor microenvironment, which is essential for advancing knowledge of breast cancer (BC). Additionally, PDX models enable the study of BC systemic effects, which is not possible using in vitro models. Traditional methods for implanting BC xenografts typically involve anesthesia and sterile surgical procedures, which are time-consuming, invasive, and limit the scalability of PDX models in BC research.
View Article and Find Full Text PDFEffects of Capsicum Oleoresin on the Energy Expenditure and Mitochondrial Content of Brown Adipose Tissue in Mice Fed a High-Fat Diet.
Prev Nutr Food Sci
December 2024
Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 03760, Korea.
Capsicum oleoresin (CO) is a concentrated extract derived from peppers ( L.) containing capsaicin (the active compound responsible for its pungency) and other bioactive components. The present study aimed to determine whether CO affects the energy expenditure and mitochondrial content of brown adipose tissue (BAT) in diet-induced obese mice.
View Article and Find Full Text PDFLncLSTA: a versatile predictor unveiling subcellular localization of lncRNAs through long-short term attention.
Bioinform Adv
November 2024
School of Information Engineering, Huzhou University, Huzhou, Zhejiang 313000, China.
Motivation: Much evidence suggests that the subcellular localization of long-stranded noncoding RNAs (LncRNAs) provides key insights for the study of their biological function.
Results: This study proposes a novel deep learning framework, LncLSTA, designed for predicting the subcellular localization of LncRNAs. It firstly exploits LncRNA sequence, electron-ion interaction pseudopotentials, and nucleotide chemical property as feature inputs.
Long-term effect of repeated application of pig slurry digestate on microbial communities in arable soils.
Heliyon
January 2025
Agroécologie, French National Institute for Agriculture, Food, and Environment (INRAE), Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, France.
Anaerobic digestion represents an opportunity for converting organic waste (OW) into valuable products: renewable energy (biogas) and a fertilizer (digestate). However, the long-term effects of digestates on soil biota, especially microorganisms, need to be better documented to understand the impact of digestate on soil ecosystem functioning and resilience. This study assessed the cumulative effect of repeated pig slurry digestate applications on soil microbial communities over a decade, using an in-situ approach to compare digested feedstock with undigested feedstock and other fertilization treatments.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!