This paper describes the implementation and optimization of a dual-confocal autofocusing system that can easily describe a real-time position by measuring the response signal (i.e., intensity) of the front and the rear focal points of the system. This is a new and systematic design strategy that would make it possible to use this system for other applications while retrieving their characteristic curves experimentally; there is even a good chance of this technique becoming the gold standard for optimizing these dual-confocal configurations. We adopt two indexes to predict our system performance and discover that the rear focal position and its physical design are major factors. A laboratory-built prototype was constructed and demonstrated to ensure that its optimization was valid. The experimental results showed that a total optical difference from 150 to 400 mm significantly affected the effective volume of our designed autofocusing system. The results also showed that the sensitivity of the dual-confocal autofocusing system is affected more by the position of the rear focal point than the position of the front focal point. The final optimizing setup indicated that the rear focal length and the front focal length should be set at 200 and 100 mm, respectively. In addition, the characteristic curve between the focus error signal and its position could successfully define the exact position by a polynomial equation of the sixth order, meaning that the system can be straightforwardly applied to an accurate micro-optical auto-focusing system.
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http://dx.doi.org/10.3390/s20123479 | DOI Listing |
Biomed Opt Express
January 2025
School of Computer Science and Technology, Hainan University, Haikou 570228, China.
Whole slide imaging (WSI) provides tissue visualization at the cellular level, thereby enhancing the effectiveness of computer-aided diagnostic systems. High-precision autofocusing methods are essential for ensuring the quality of WSI. However, the accuracy of existing autofocusing techniques can be notably affected by variations in staining and sample heterogeneity, particularly without the addition of extra hardware.
View Article and Find Full Text PDFWhen observing chip-to-free-space light beams from silicon photonics (SiPh) to free space, manual adjustment of camera lens is often required to obtain a focused image of the light beams. In this Letter, we demonstrated an auto-focusing system based on the you-only-look-once (YOLO) model. The trained YOLO model exhibits high classification accuracy of 99.
View Article and Find Full Text PDFAutofocusing beams have attracted widespread attention due to their advantages in optical trapping, but their propagation behavior in complex optical systems is still unclear. Here, we obtain the analytical propagation formulas for autofocusing beams through optical systems described by ABCD matrices. Foci adjustment through a lens and oscillate behavior in a parabolic potential medium of the beams are discussed.
View Article and Find Full Text PDFBased on a split-step Fourier algorithm, the transmission of circular Airy beams with quadratic phase modulation (QPM) is investigated in the fractional Schrödinger equation (FSE) under diffraction modulations (periodic modulation, linear modulation and power function modulation) and external potentials (parabolic potential and linear potential). The results show that QPM is able to change the focusing position and intensity, as well as the transmission trajectory of the beam. In a periodic modulation, the circular Airy beam (CAB) exhibits periodic variation characteristics, and the beam splitting is retarded under the action of the QPM.
View Article and Find Full Text PDFThe rapid increase in orbital angular momentum (OAM) mode size with increasing modal order, given the limited-size of the receiver, is a major impediment to high-capacity OAM mode multiplexing in practice. Based on the Pancharatnam-Berry (PB) phase theory, we correlate the change of the polarization state with the curvature of the wavefront isophase line in the source plane and manipulate the focusing ability of vector autofocusing Airy vortex beam (AAVB) by combining the isophase line curvature and the intensity gradient of the beam, which are two independent degrees of freedom. The present method enables flexible on-demand focusing of vector AAVBs in free space and is more effective in focusing higher order OAM modes, which can reduce the full-width at half maximum (FWHM) of the AAVB with topological charge l= 25 to 1/5 of that of the conventional scalar type.
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