AI Article Synopsis
- Essential oils (EOs) are effective against a variety of microorganisms, and this study used a microarray approach to analyze gene expression in fungi exposed to the vapors of eight antifungal EOs.
- The analysis revealed that, out of 12,675 genes, only 551 were annotated, while the rest were hypothetical, with 1,350 genes being upregulated and 765 downregulated when exposed to at least four EOs.
- This research is significant as it marks the first instance of using microarray hybridization to study gene expression in fungi affected by different essential oils, highlighting the impact of EOs on metabolic processes vital to the organism.
Article Abstract
Essential oils (EOs) are well-known for their beneficial properties against a broad range of microorganisms. For the better understanding of their mechanism of action in fungi, a microarray approach was used in order to evaluate the gene expression of (recently renamed ) exposed to the indirect contact (vapors) of eight EOs. The selection of assayed EOs was based on their antifungal activity. The extraction of RNA and the microarray hybridization procedure were optimized for the analysis of Gene ontology annotation was performed to investigate the functional analysis of the genes. To uncover the metabolic pathway of these differentially expressed genes, they were mapped into the KEGG BRITE pathway database. The transcriptomic analysis showed that, from a total of 12,675 genes, only 551 genes are annotated, and the other 12,124 genes encoded hypothetical proteins. Further bioinformatic analysis demonstrated that 1350 genes were upregulated and 765 downregulated at least with half (four) of the utilizing EOs. A microarray investigation has confirmed the main impact of EOs to metabolic processes in involved in vital functions. Presumably, this is the first time that a microarray hybridization analysis was performed in order to evaluate the gene expression of exposed to various EOs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345231 | PMC |
| http://dx.doi.org/10.3390/antibiotics9060343 | DOI Listing |
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