Effect of silkworm () pupae oil supplementation on enteric methane emission and methanogens diversity in sheep.

and studies were conducted to examine the effect of silkworm pupae oil on methane (CH) emission and methanogens diversity. Five graded levels (2, 4, 6, 8 and 10%) of silkworm pupae oil were tested . Eighteen adult sheep were divided into three groups. All the animals were fed on similar basal diet except the oil supplementation in test groups. Oil level for supplementation was decided on the basis of study. study indicated a reduction of 22% in CH production with 2% oil supplementation. Animals in test groups were supplemented with oil (2%) either daily (CON) or intermittently (INT) on every alternate week for all the seven days. A significant reduction of 17-20% in enteric CH emission (g/d) was achieved due to oil supplementation in sheep. However, No variation was established between test groups CON and INT. In present study, was major genus contributed ∼90% of the total rumen methanogens; whilst was the most abundant methanogens species. Abundance of was affected with the oil supplementation. It can be concluded that the silkworm pupae oil at 2% can decrease CH emission by 15-20%.