Genetically encodable calcium ion (Ca) indicators (GECIs) based on green fluorescent proteins (GFP) are powerful tools for imaging of cell signaling and neural activity in model organisms. Following almost 2 decades of steady improvements in the GFP-based GCaMP series of GECIs, the performance of the most recent generation (i.e., jGCaMP7) may have reached its practical limit due to the inherent properties of GFP. In an effort to sustain the steady progression toward ever-improved GECIs, we undertook the development of a new GECI based on the bright monomeric GFP, mNeonGreen (mNG). The resulting indicator, mNG-GECO1, is 60% brighter than GCaMP6s in vitro and provides comparable performance as demonstrated by imaging Ca dynamics in cultured cells, primary neurons, and in vivo in larval zebrafish. These results suggest that mNG-GECO1 is a promising next-generation GECI that could inherit the mantle of GCaMP and allow the steady improvement of GECIs to continue for generations to come.
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http://dx.doi.org/10.1021/acssensors.0c00279 | DOI Listing |
Optical metasurfaces offer significant advantages in enhancing the speed, efficiency, and miniaturization of imaging systems. However, most existing metasurfaces are limited to static functionalities and lack reconfigurability, which is a key feature for practical applications in dynamic environments. In this work, we demonstrate a reconfigurable optical metasurface capable of switching between two distinct imaging functions (edge detection and bright-field imaging) within the visible spectrum.
View Article and Find Full Text PDFNat Commun
January 2025
Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden.
One of the key advantages of perovskite light-emitting diodes (PeLEDs) is their potential to achieve high performance at much higher current densities compared to conventional solution-processed emitters. However, state-of-the-art PeLEDs have not yet reached this potential, often suffering from severe current-efficiency roll-off under intensive electrical excitations. Here, we demonstrate bright PeLEDs, with a peak radiance of 2409 W sr m and negligible current-efficiency roll-off, maintaining high external quantum efficiency over 20% even at current densities as high as 2270 mA cm.
View Article and Find Full Text PDFAdv Mater
January 2025
Fujian Key Laboratory of Surface and Interface Engineering for High Performance Materials, College of Materials, Xiamen University, Xiamen, 361005, P. R. China.
Quantum dot (QD)-converted micrometer-scale light-emitting diodes (micro-LEDs) are regarded as an effective solution for achieving high-performance full-color micro-LED displays because of their narrow-band emission, simplified mass transfer, facile drive circuits, and low cost. However, these micro-LEDs suffer from significant blue light leakage and unsatisfactory electroluminescence properties due to the poor light conversion efficiency and stability of the QDs. Herein, the construction of green and red QD luminescence microspheres with the simultaneously high conversion efficiency of blue light and strong photoluminescence stability are proposed.
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January 2025
Department of Chemistry, Hanyang University, Seoul, Republic of Korea.
Mechanoluminescence platforms, combining phosphors with elastic polymer matrix, have emerged in smart wearable technology due to their superior elasticity and mechanically driven luminescent properties. However, their luminescence performance often deteriorates under extreme elastic conditions owing to a misinterpretation of polymer matrix behavior. Here, we unveil the role of the polymer matrices in mechanoluminescence through an interface-triboelectric effect driven by elasticity, achieving both high elasticity and brightness.
View Article and Find Full Text PDFJ Med Chem
January 2025
Center for Advanced Materials Research & Faculty of Arts and Sciences, Beijing Normal University, Zhuhai 519087, China.
Fluorescence molecular imaging aims to enhance clarity in the region of interest, particularly in the near-infrared IIb window (NIR-IIb, 1500-1700 nm). To achieve this, we developed a novel small-molecule dye, named , based on classic cyanine dyes (heptamethine or pentamethine is essential for wavelengths beyond 1000 nm). By reducing excessive polymethine to a single methine and disrupting symmetry to form an asymmetric donor-π-acceptor (D-π-A) architecture, we enhanced the donor's electron-donating capability, yielding emission at 1088 nm.
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