The lipopolysaccharide was obtained from the cells of Azospirillum formosense CC-Nfb-7(T), a diazotrophic bacterium isolated from agricultural soil. The O-specific polysaccharide (OPS) was released by mild acid hydrolysis of the lipopolysaccharide and was studied by sugar analysis along with H and C NMR spectroscopy, including H,H COSY, TOCSY, ROESY, H,C HSQC, and HMBC experiments, and Smith degradation. The following structure of partially methylated OPS composed of trisaccharide repeating units was established.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.carres.2020.108060 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Some Human Anti-Glycan Antibodies Lack the Ability to Activate the Complement System.
Antibodies (Basel)
December 2024
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Science, 117991 Moscow, Russia.
Naturally occurring human antibodies against glycans recognize and quickly eliminate infectious bacteria, viruses and aberrantly glycosylated neoplastic malignant cells, and they often initiate processes that involve the complement system. Using a printed glycan array (PGA) containing 605 glycoligands (oligo- and polysaccharides, glycopeptides), we examined which of the glycan-binding antibodies are able to activate the complement system. Using this PGA, the specificities of antibodies of the IgM and IgG classes were determined in the blood serum of healthy donors (suggested as mostly natural), and, then, using the same array, it was determined which types of the bound immunoglobulins were also showing C3 deposition.
View Article and Find Full Text PDFPhage-resistance alters Lipid A reactogenicity: a new strategy for LPS-based conjugate vaccines against Rissen.
Front Immunol
December 2024
Department of Agricultural Sciences, University of Naples Federico II, Portici, Italy.
serovar Rissen ( Rissen) is an emerging causative agent of foodborne diseases. The current emergence of antibiotic resistance makes necessary alternative therapeutic strategies. In this study, we investigated the potential of a phage-resistant strain of Rissen (R) as a tool for developing an effective lipopolysaccharide (LPS)-based vaccine.
View Article and Find Full Text PDFImmunological studies of Burkholderia multivorans O-antigen oligosaccharide-rsScpA193 conjugates as potential candidates for vaccine development.
Int J Biol Macromol
December 2024
National Glycoengineering Research Center and NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-based Medicine, Shandong University, 72 Binhai Road, Qingdao 266237, China. Electronic address:
The Burkholderia cepacia complex (Bcc) is a group of phenotypically similar but genotypically diverse Gram-negative bacteria that pose a significant threat to public health worldwide. Due to the absence of effective therapies, the development of an effective vaccine against Bcc infection is urgently needed. Lipopolysaccharide (LPS) O-antigens of B.
View Article and Find Full Text PDFSerotype switching in Pseudomonas aeruginosa ST111 enhances adhesion and virulence.
PLoS Pathog
December 2024
Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs. Lyngby, Denmark.
Evolution of the highly successful and multidrug resistant clone ST111 in Pseudomonas aeruginosa involves serotype switching from O-antigen O4 to O12. How expression of a different O-antigen serotype alters pathogen physiology to enable global dissemination of this high-risk clone-type is not understood. Here, we engineered isogenic laboratory and clinical P.
View Article and Find Full Text PDFMolecular Epidemiology and Antimicrobial Resistance of Klebsiella pneumoniae Strains Isolated From Dairy Cows in Xinjiang, China.
Vet Med Sci
January 2025
College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
Background: Klebsiella pneumoniae is an opportunistic pathogen that causes severe infections in humans and animals. Nonetheless, little is known about the molecular epidemiology of mastitis-associated K. pneumoniae in dairy cows.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!