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mRNA-Based Reprogramming Under Xeno-Free and Feeder-Free Conditions. | LitMetric

mRNA-Based Reprogramming Under Xeno-Free and Feeder-Free Conditions.

Methods Mol Biol

Stem Cell Laboratory, Assisted Conception Unit, School of Life Course Sciences, Department of Women and Children's Health, Faculty of Life Sciences & Medicine, King's College London, London, UK.

Published: June 2022

AI Article Synopsis

  • Reprogramming somatic cells into induced pluripotent stem cells (iPSCs) has advanced tissue engineering, regenerative medicine, and cell therapy.
  • There's a growing demand for efficient and less labor-intensive methods of reprogramming that are xeno-free, feeder-free, and chemically defined.
  • The StemRNA 3rd Gen Reprogramming Kit from ReproCELL offers a new method using non-modified microRNAs and RNAs, enhancing the efficiency of generating iPSCs.

Article Abstract

Reprogramming somatic cells into induced pluripotent stem cells (iPSC) has provided a gateway for many novel discoveries in the field of tissue engineering, regenerative medicine and cell therapy. The need for an efficient, less laborious and fast reprogramming protocol under xeno-free, feeder-free and chemically defined conditions has never been greater. Here we describe a novel approach to reprogramming using the StemRNA 3rd Gen Reprogramming Kit (ReproCELL) which encompasses non-modified microRNAs (NM-miRNA), non-modified E3, K3, B18R RNAs (EKB NM-RNA) and non-modified mRNAs for six crucial transcription factors (OSKMNL NM-RNA).

Download full-text PDF

Source
http://dx.doi.org/10.1007/7651_2020_302DOI Listing

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