Background: Vulnerable plaques have been generally recognized to play a role in the pathogenesis of acute myocardial infarction (AMI), however, the role of circulating CX3CR1CD163 M2 monocytes has not been studied properly. We aim to evaluate the features of CX3CR1CD163 M2 monocytes and its relationship with cardiac specific markers in AMI patients.
Methods: The circulating M2 monocytes were identified in AMI patients (n=35) and healthy controls (HCs, n=10) by flow cytometry using two staining methods: CD68CD163 (cytoplasmic staining) and CX3CR1CD163 (surface staining). CX3CR1 monocytes were purified by magnetic cell sorting. The expression level of peroxisome proliferator-activated receptor γ (PPARγ) and arginase-1 (Arg-1) were measured by real-time quantitative PCR and Western Blot in CX3CR1 monocytes.
Results: Circulating M2 monocytes extremely expanded in AMI patients compared with HCs (P<0.01). Positive linear correlation was confirmed between CD68CD163 and CX3CR1CD163 cell populations in AMI patients (r=0.39, P=0.02). The percentage of circulating CX3CR1CD163 M2 monocytes positively correlated with cardiac specific markers (cTNT, CK-MB) and acute phase markers (glucose, hs-CRP) (cTNT, r=0.63, P<0.01, CK-MB, r=0.54, P<0.01, glucose, r=0.62, P<0.01, hs-CRP, r=0.58, P<0.01). CX3CR1 monocytes in AMI patients expressed higher levels of PPARγ and Arg-1 than those in HCs (P<0.01).
Conclusions: Circulating M2 monocytes increased in AMI patients and positively correlated with the elevation of both cardiac specific and acute phase markers. CX3CR1CD163 M2 monocytes might have application value for the early diagnosis of AMI.
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| http://dx.doi.org/10.21037/atm-20-383 | DOI Listing |
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