Transcriptomics reveal high regulatory diversity of drought tolerance strategies in a biennial oil crop.

Spring droughts are expected to become more frequent in Central Europe as a result of climate change. Their coincidence with flowering of biennial crops like winter oilseed rape (Brassica napus) can cause major impact for yield development. However, no data is available on the diversity of genetic regulation of drought tolerance during this stage under realistic conditions. Here, we assessed the phenotypic plasticity of drought response for eight diverse B. napus accessions under field-like conditions and linked their stress response to gene and miRNA expression during early and late stress. We observed highly diverse responses, both on the phenotypic and on the gene expression level. Our data suggest that drought tolerant accessions have more effective molecular protection mechanisms like ROS scavenging, source/sink ratio and regulation of developmental timing, compared to otherwise phenotypically similar accessions. Bna.MAP3K13.C05 expression was found to be protective independently of the tolerance mechanism, indicating cross-talk to nitrogen signaling. Moreover, we identified putative miRNA genes in the B. napus genome which respond to stress and may also be involved in protective mechanisms, representing possible breeding targets.