sp. nov., isolated from the roots of rice.

A novel Gram-stain-negative strain, designated ZYY5, was isolated from rice roots. Results of 16S rRNA gene analysis indicated that strain ZYY5 was a member of the genus , with a highest similarity to DSM 18068 (98.5%). The major fatty acids were summed feature 3 (C ω7 and/or C ω6), C and summed feature 8 (C ω7 and/or C ω6). Multi-locus sequence analysis using five concatenated genes (16S rRNA, , , and ) and phylogenomic analysis based on 2940 core gene sequences showed that strain ZYY5 formed a robust cluster with strains EC1, ZJU1202, DZ2Q, NCPPB 3531 and CSL RW192, while separated from the other strains of . The orthologous average nucleotide identity (ANI) and digital DNA-DNAhybridization (dDDH) values among these six strains ranged from 96.8-99.9% and 73.7-99.8%, which supported that they were belonged to the same species. However, strain ZYY5 shared 58.4 of dDDH and 94.5% of ANI values with type strain DSM 18068, which were lower than the proposed species boundary cut-off for dDDH and ANI. The genomic analysis revealed that strain ZYY5 contained virulence-associated genes, which is same as the phylogenetic-related strains of the genus . Based on the results of the polyphasic approaches, we propose that strain ZYY5 represents a novel species in the genus , for which the name sp. nov. (=JCM 33020 =ACCC 61554 ) is proposed. Strains EC1, ZJU1202, DZ2Q, NCPPB 3531 and CSL RW192 should also be classified in the same genomospecies of same as ZYY5.