AI Article Synopsis
- The study focused on the anticancer effects of Boiss. essential oil on human colorectal cancer cell lines (HCT116 & SW48).
- It evaluated the oil's cytotoxicity and ability to induce apoptosis using various assays and flow cytometry to measure reactive oxygen species (ROS).
- The findings indicate that the essential oil significantly inhibits cell growth and induces cell death through a specific mitochondrial pathway, suggesting its potential as a colorectal cancer treatment.
Article Abstract
Background: Boiss. is known by the common Persian name "", is one of the best-known medicinal herbs belonging to the Labiatae (Lamiaceae) family. The aim of this study was to evaluate the anticancer effects and the underlying mechanisms of how Boiss., essential oil induced apoptosis in the human colorectal tumor cell lines (HCT116 & SW48).
Methods: This study was conducted in National Institute of Genetic Engineering and Biotechnology (NIGEB) (Tehran, Iran) from 2017 to 2019. The cytotoxicity of this essential oil was assessed by 3- (4,5-di-methylthiazol-2-yl)- 2,5-diphenyltetra-zolium bromide MTT) assay, trypan blue exclusion, and colony formation assays. We assessed apoptosis and measure intracellular reactive oxygen species (ROS) by flow cytometry. Then gene expression was analyzed by Quantitative Real-Time RT-PCR.
Results: Boiss., essential oil time- and dose-dependently inhibits cell proliferation and also induced apoptosis in both cell lines via UCP-related mitochondrial pathway by the induction of intracellular ROS.
Conclusion: Boiss., essential oil could be a good candidate for use as an inhibitor of the growth of colorectal tumor cells.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283189 | PMC |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!