Exposition to Biological Control Agent Increases the Development of Cancer in Mice Injected With Murine Melanoma.

Biological control agents (BCA) are an alternative to chemical pesticides and an emerging strategy to safely eliminate plant pathogens. spp. are the most common fungi used as BCAs. They produce spores that are released into the air and can potentially interact with immune system of mammals. We previously showed that affects expression of genes encoding pattern recognition receptors (PRRs) and cytokines in mice. PRRs are involved in the recognition of microorganisms and can lead to pro-tumoral signaling. Here, we evaluated if mice injected with low doses of murine melanoma exhibited increased development of lung tumor when treated with conidia of . Mice treated with and inoculated with B16-F10 melanoma cells exhibited significant increase in tumor uptake ( = 0.006) and increased number of visible nodules in the lungs ( = 0.015). We also analyzed mRNA expression levels of genes encoding PRRs in lung of mice exposed to and demonstrated that mice treated with conidia exhibited lower expression levels of and increased expression of (toll like receptor 4) compared to non-treated controls. The expression levels of and were increased in mice treated with and inoculated with murine melanoma compared to controls only inoculated with melanoma. Our results demonstrate that intranasal exposition to increases tumor in the B16-F10 model, which may raise concerns regarding the safety of its use in agriculture.