Objective: The primary purpose of this study was to evaluate and compare the microbial loads and pathogenicity traits of oral in denture-wearing (DW; = 15) and nondenture-wearing (NDW; = 15) elderly persons.
Materials And Methods: The fungal counts of the saliva, tongue dorsa, and prosthesis-fitting surfaces of the participants were assessed using real-time polymerase chain reaction to compare the quantity and expression of selected biofilm-associated genes (, , and ).
Statistical Analysis: The obtained data were analyzed by one-way analysis of variance, followed by Bartlett's test. When appropriate, the Student's -test was also used; a value of < 0.05 was considered statistically significant.
Results: In both groups, the count of was found to be significantly higher in saliva than in other oral samples. The expression of the hypha-specific genes ( and ) in the tongue dorsa was higher in the DW group ( < 0.05), whereas the transcription level of the yeast-specific gene ( was significantly higher in the NDW group.
Conclusion: Both tongue dorsa and dentures appear to be sharing factors that are important for biofilm growth in abiotic and biotic oral surfaces of the elderly.
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| http://dx.doi.org/10.1055/s-0040-1712779 | DOI Listing |
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