In the current study we investigated the inhibitory effect of rucaparib (Rubraca) on human ovarian cancer SKOV3 and A2780 cells and its possible mechanism. Cancer cells and human normal ovarian epithelial IOSE80 cells were treated with Rubraca at different concentrations. Cell viability was measured by MTT assay. Necrotizing apoptosis was detected by Annexin V-FITC/PI double staining combined with flow cytometry. Reactive oxygen species were measured by 2',7'-dichlorofluorescent yellow diacetate (DCFH-DA) fluorescent probe. The expression of receptor-interacting protein kinase 1 (RIP1) and RIP3 protein was determined by Western Blot. Our data showed that Rubraca inhibited the proliferation of ovarian cancer SKOV3 and A2780 cells in a dose-and time-dependent manner. After Rubraca treatment, the apoptotic rate of SKOV3 and A2780 cells (Annexin V+/PI-cells) did not change significantly, but the proportion of necrotic cells (PI+cells or Annexin V+/PI+cells) increased significantly, which was different from the control group. Furthermore, Rubraca could significantly induce SKOV3 and A2780 cells to produce excessive reactive oxygen species and significantly upregulate the expression of RIP1 and RIP3. When pretreated with reactive oxygen species inhibitor N-acetyl-L-cysteine (NAC) or RIP1 inhibitor (Nec-1), the necrosis apoptotic rate of SKOV3 and A2780 cells decreased significantly. In summary, Rubraca could significantly inhibit the proliferation of ovarian cancer SKOV3 and A2780 cells, which may be partially achieved upregulating the expression of RIP1 and RIP3 proteins, and activating the process of necrotic apoptosis.
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http://dx.doi.org/10.1691/ph.2020.9827 | DOI Listing |
BMC Cancer
December 2024
Department of Gynecology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University, Peking University Cancer Hospital Yunnan, Kunming, 650118, Yunnan, P. R. China.
New treatment strategies for ovarian cancer, which is the deadliest female reproductive tract malignancy, are urgently needed. Here, we investigated the anticancer effects of fenbendazole (FBZ), a benzimidazole compound, on the regulation of apoptosis and mitotic catastrophe in A2780 and SKOV3 human epithelial ovarian cancer cells. Functional experiments, including Cell Counting Kit 8 (CCK-8), colony formation, and flow cytometry assays, were conducted to explore the effects of FBZ on the malignant biological behavior of A2780 and SKOV3 cells.
View Article and Find Full Text PDFFront Chem
December 2024
Department of Medical Laboratory Sciences, College of Applied Medical Laboratory Sciences, Majmaah University, Al Majma'ah, Saudi Arabia.
Lotus seeds, also known as Nelumbinis semen, has been utilized for over 7,000 years as vegetable, functional food and medicine. In this study, we primarily investigated the anticancer effects of lotus seed extracts, particularly of the methanolic extract (MELS) on cell proliferation inhibition, apoptosis induction and cell cycle arrest in ovarian cancer cell lines. Further, we studied the phytochemical composition of the MELS by gas chromatography-mass spectrometry (GC-MS) analysis.
View Article and Find Full Text PDFFront Oncol
December 2024
School of Life Sciences, Qilu Normal University, Jinan, China.
Background: Ovarian cancer (OV) is a common malignancy in the female reproductive system, characterized by poor prognosis and high recurrence rates. The discovery of dependable molecular markers is crucial for improving the timeliness of detection, diagnosis, and treatment, ultimately aiming to lower fatality rates. CNNM4 (cyclin and CBS domain divalent metal cation transport mediator 4), a member of the CNNM (Cyclin M) family, binds to PRL (prolactin) to regulate magnesium homeostasis and influence tumor cell proliferation.
View Article and Find Full Text PDFCurr Med Chem
December 2024
Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa 31982, Saudi Arabia.
Introduction: A series of benzylidene derivatives of fenobam and its thio analogues (1-22) have been evaluated for their cytotoxicity against breast cancer (MCF-7, MDA-MB-231), ovarian cancer (A2780, SKOV-3) and cervical cancer (HELA) cell lines.
Method: These compounds (1-22) exhibited 72-83% inhibition of Erk activity against the ovarian cancer cell line (A2780). Compounds 3 and 20 showed the highest DNA damage effect in Comet Assay against the A2780 cancer cell line as compared to the other tested analogues (4, 8, 11, 12, and 13) by using % Tail DNA and OTM.
BMB Rep
December 2024
Hicelltech Inc., Yangsan 50612, Korea; Department of Physiology, School of Medicine, Pusan National University, Yangsan 50612, Korea.
Ovarian cancer is the deadliest gynecological cancer because it has few early symptoms and metastasizes to the surrounding organs at advanced stages. Cancer stem cells (CSCs), a subpopulation of cells with acquired drug resistance, contribute to the recurrence and poor prognosis of ovarian cancer. CD109, a cell surface glycoprotein, has been reported to be a marker of CSCs; however, it remains unclear whether CD109 is secreted by CSCs.
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