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* The study aimed to use flow cytometry to analyze blood samples from patients with three types of primary humoral immunodeficiencies.
* Results indicated significant decreases in B lymphocytes in X-linked agammaglobulinemia, absence of CD154 in hyper-immunoglobulin M syndrome, and varied B lymphocyte subpopulations in common variable immunodeficiency, highlighting flow cytometry's utility for early diagnosis.
Article Abstract
Background: Antibody deficiencies encompass a wide spectrum of pathologies and constitute approximately 50 % of primary immunodeficiencies; with cytometry, it is possible to evaluate the immune status rapidly, effectively and at low cost.
Objective: To assess, by means of flow cytometry, the cells of patients with three types of primary humoral immunodeficiencies.
Method: Using flow cytometry, blood samples from patients and healthy subjects were analyzed with different monoclonal antibodies.
Results: Using various stains, a severe decrease in B lymphocytes was shown in patients with X-linked agammaglobulinemia, as well as a lack of CD154 expression in patients with hyper-immunoglobulin M syndrome, and heterogeneity of B lymphocyte subpopulations in patients with common variable immunodeficiency.
Conclusion: Flow cytometry enables early diagnosis of primary immunodeficiencies with a high level of confidence and, in many cases, identification of the genes involved.
