AI Article Synopsis
- A new analytical method for detecting oxcarbazepine in human plasma and urine samples is presented, utilizing a vortex-assisted switchable hydrophilicity solvent-based extraction.
- The method incorporates optimization of parameters like solvent volume, sodium hydroxide concentration, and vortex timing, achieving a detection range of 27.03 to 353.47 μg/kg.
- Results show low limits of detection (6.2 μg/kg) and high recovery rates (97-100%) for spiked sample tests, indicating the method's reliability and effectiveness.
Article Abstract
This work presents a sensitive and rapid analytical method for the determination of oxcarbazepine in human plasma and urine samples. A vortex-assisted switchable hydrophilicity solvent-based liquid phase microextraction (VA-SHS-LPME) was used to preconcentrate oxcarbazepine from the samples before the determination by gas chromatography mass spectrometry. The switchable hydrophilicity solvent was synthesized by protonating N,N-dimethylbenzylamine with carbon dioxide to make it totally miscible with an equivalent volume of water. Parameters of the VA-SHS-LPME method including volume of switchable hydrophilicity solvent, concentration/volume of sodium hydroxide and vortex period were systematically optimized. Under the optimum conditions, good linearity ranging from 27.03 to 353.47 μg/kg was obtained for the analyte. Limit of detection and quantitation values were found to be 6.2 and 21 μg/kg (mass base), respectively. The relative standard deviation was calculated as 6.9% for six replicate measurements of the lowest concentration of the calibration plot. Satisfactory recovery results were calculated in the range of 97-100% for human plasma and urine samples spiked at five different concentrations.
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| http://dx.doi.org/10.1002/bmc.4915 | DOI Listing |
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