The dynamin-related proteins (DRPs) are self-assembling membrane remodeling machines that are indispensable for fundamental cellular trafficking and homeostatic processes. We describe in this chapter protocols developed in our laboratory for purification of full-length and minimal constructs of Chaetomium thermophilum Vps1, the model fungal DRP, using mammalian and Escherichia coli expression systems.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-0676-6_2 | DOI Listing |
Publication Analysis
Top Keywords
expression systems
8
purification dynamin-related
4
dynamin-related protein
4
protein vps1
4
vps1 mammalian
4
mammalian bacterial
4
bacterial expression
4
systems dynamin-related
4
dynamin-related proteins
4
proteins drps
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!