The dynamics of phosphomonoesters, phosphodiesters, Pi, ATP, ADP, NAD(H+) and uridine diphosphoglucose (UDPG) levels in rat liver upon sharp oscillations in the rates of protein and nucleic acid biosynthesis induced by a sublethal++ dose of cycloheximide was studied, using the 31P-NMR method. The results obtained with preparations of native liver are unaffected by fractionation, homogenization and chemical extraction procedures. It was demonstrated that oscillations of Pi, ATP and UDPG levels in liver cells reflect the changes in the energy consumption and intracellular energy-linked processes (e.g., glycolysis, oxidative phosphorylation, glycogen synthesis and consumption) under conditions of variable macromolecular synthesis rates. The oscillations in phosphomonoesters and phosphodiesters levels are mainly due to cycloheximide-induced lipid metabolism disturbances.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Mapping Age Differences in Brain Energy Metabolites and Metabolic Markers of Cellular Membrane Production and Degradation With P Magnetic Resonance Spectroscopy.
Hum Brain Mapp
October 2024
Department of Psychiatry and Behavioral Neurosciences, Wayne State University, Detroit, Michigan, USA.
Article Synopsis
- The study used Phosphorus Magnetic Resonance Spectroscopy (P MRS) to analyze brain metabolites related to energy and cell membrane production in children and adults across various age groups.
- Results showed that children had higher phosphomonoesters (PMEs) and γαATP levels, while adults had more phosphodiesters (PDEs) and lower intracellular pH, indicating different metabolic profiles based on age.
- These findings suggest that as individuals age, there's a reduction in membrane synthesis and energy consumption, with a notable decline in metabolic processes occurring from childhood to older adulthood.
P multi-echo MRSI with low B dual-band refocusing RF pulses.
NMR Biomed
January 2025
Center for Image Sciences, University Medical Center Utrecht, Utrecht, The Netherlands.
P magnetic resonance spectroscopy (MRS) can spectrally resolve metabolites involved in phospholipid metabolism whose levels are altered in many cancers. Ultra-high field facilitates the detection of phosphomonoesters (PMEs) and phosphodiesters (PDEs) with increased SNR and spectral resolution. Utilizing multi-echo MR spectroscopic imaging (MRSI) further enhances SNR and enables T information estimation per metabolite.
View Article and Find Full Text PDFNeuroenergetic alterations in neurodegenerative diseases: A systematic review and meta-analysis of in vivoP-MRS studies.
Ageing Res Rev
November 2024
Department of Neurology, RWTH Aachen University, Aachen, Germany; JARA-Brain Institute Molecular Neuroscience and Neuroimaging (INM-11), Research Centre Jülich and RWTH Aachen University, Aachen, Germany. Electronic address:
Phosphorus magnetic resonance spectroscopy (P-MRS) is applied for non-invasive studies of neuroenergetic metabolism in neurodegenerative diseases. However, the findings are inconsistent and have not yet been tested in meta-analyses. To address this gap, we performed a systematic review of 29 studies and conducted meta-analyses for 9 studies on Alzheimer's disease (AD, n = 140 patients), 9 studies on Parkinson's disease (PD, n = 183 patients), 3 studies on Progressive Supranuclear Palsy (PSP, n = 42 patients), and 2 studies on Multiple System Atrophy (MSA, n = 24 patients).
View Article and Find Full Text PDFMeasurement of metabolite levels and treatment-induced changes in hepatic metastases of gastro-esophageal cancer using 7-T phosphorus magnetic resonance spectroscopic imaging.
NMR Biomed
September 2024
Center for Image Sciences, University Medical Center Utrecht, Utrecht, The Netherlands.
Methods for early treatment response evaluation to systemic therapy of liver metastases are lacking. Tumor tissue often exhibits an increased ratio of phosphomonoesters to phosphodiesters (PME/PDE), which can be noninvasively measured by phosphorus magnetic resonance spectroscopy (P MRS), and may be a marker for early therapy response assessment in liver metastases. However, with commonly used P surface coils for liver P MRS, the liver is not fully covered, and metastases may be missed.
View Article and Find Full Text PDFMultiparametric Aging Study Across Adulthood in the Leg Through Quantitative MR Imaging, H Spectroscopy, and P Spectroscopy at 3T.
J Magn Reson Imaging
January 2025
NMR Laboratory, Neuromuscular Investigation Center, Institute of Myology, Paris, France.
Background: Improved characterization of healthy muscle aging is needed to establish early biomarkers in age-related diseases.
Purpose: To quantify age-related changes on multiple MRI and clinical variables evaluated in the same cohort and identify correlations among them.
Study Type: Prospective.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!