Transgenic immediate-early gene reporter mouse strains are valuable tools for studying activity-dependent neural cell populations in vivo. However, routine characterization of the Gene Expression Nervous System Atlas (GENSAT) "Egr1-EGFP" reporter mouse strain produced results that were highly inconsistent with endogenous Egr1 expression. Activity-dependent EGFP expression was not observed, and EGFP protein did not co-localize with native Egr1 protein. This precautionary study outlines the limitations of the Egr1-EGFP transgenic line as a tool to study the activity-dependent expression of Egr1 and emphasizes the necessity of taking into account the potential loss of regulatory elements, stability determinants, or translational modulation in transgenic reporter strains.
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Egr1-EGFP transgenic mouse allows in vivo recording of Egr1 expression and neural activity.
J Neurosci Methods
November 2021
School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China. Electronic address:
Background: Immediate-early genes (IEGs) have been serving as markers of active neurons for their rapid responses to stimulation. With the development of IEG-EGFP reporters by the GENSAT project, application of the IEGs have been greatly expanded. However, detailed validations for these systems are still lacking, causing trouble in the interpretation of the fluorescence signals.
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View Article and Find Full Text PDFLimitations of the GENSAT Egr1-EGFP transgenic mouse strain for neural circuit activity mapping.
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Canadian Centre for Behavioural Neuroscience, University of Lethbridge, T1K 3M4, Canada; Center for Neurobiology of Learning and Memory, Department of Neurobiology and Behavior, University of California Irvine, 92697, United States of America. Electronic address:
Transgenic immediate-early gene reporter mouse strains are valuable tools for studying activity-dependent neural cell populations in vivo. However, routine characterization of the Gene Expression Nervous System Atlas (GENSAT) "Egr1-EGFP" reporter mouse strain produced results that were highly inconsistent with endogenous Egr1 expression. Activity-dependent EGFP expression was not observed, and EGFP protein did not co-localize with native Egr1 protein.
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