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Biosynthesis of Phenolic Compounds of After Inoculation with Selected PGPR Strains.
Int J Mol Sci
November 2024
Institute of Biology, University of Szczecin, Wąska 13, 71-415 Szczecin, Poland.
The phenylpropanoid biosynthesis pathway is involved in the response of plants to stress factors, including microorganisms. This paper presents how free-living strains of rhizobacteria KK5, KK7, KK4, and the symbiotic strain KK13 affect the expression of genes encoding phenylalanine ammonia-lyase (PAL), the activity of this enzyme, and the production of phenolic compounds in . Seedlings were inoculated with rhizobacteria, then at T0, T24, T72, and T168 after inoculation, the leaves and roots were analyzed for gene expression, enzyme activity, and the content of phenolic compounds.
View Article and Find Full Text PDFThe low enoyl-acyl carrier protein reductase activity of FabI2 is responsible for the high unsaturated fatty acid composition in Sinorhizobium meliloti.
BMC Microbiol
December 2024
Guangdong Provincial Key Laboratory for the Development Biology and Environmental Adaptation of Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong, 510642, China.
Background: Sinorhizobium meliloti is noted for its exceptional capacity to produce unsaturated fatty acids (UFAs). Earlier studies have indicated that S. meliloti primarily employs the FabA-FabB pathway for UFA synthesis, however, the mechanisms remain elusive.
View Article and Find Full Text PDFMicrobes display broad diversity in cobamide preferences.
bioRxiv
November 2024
Department of Plant & Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, U.S.A.
Cobamides, the vitamin B (cobalamin) family of cofactors, are used by most organisms but produced by only a fraction of prokaryotes, and are thus considered key shared nutrients among microbes. Cobamides are structurally diverse, with multiple different cobamides found in most microbial communities. The ability to use different cobamides has been tested for several bacteria and microalgae, and nearly all show preferences for certain cobamides.
View Article and Find Full Text PDFThis study aimed to identify and characterize actinobacteria and rhizobia with plant growth-promoting (PGP) traits from chickpea plants. Out of 275 isolated bacteria, 25 actinobacteria and 5 chickpea rhizobia showed 1-aminocyclopropane-1-carboxylate deaminase (ACCd) activity. Selected chickpea rhizobia were tested for their nodulating capacity under sterile and non-sterile soil conditions.
View Article and Find Full Text PDFRecent Advances in Genetic Engineering Strategies of .
ACS Synth Biol
November 2024
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
Article Synopsis
- The text discusses a soil bacterium that helps legumes fix nitrogen and has potential for producing valuable nutrients like vitamin B.
- It highlights advancements in gene editing technologies that can enhance the bacterium's traits for biotechnological uses.
- The review focuses on different genetic engineering methods, including Tn5 strategies and CRISPR/Cas systems, aiming to inspire further research on this bacterium and related species.
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