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Exploring the genomic and proteomic variations of SARS-CoV-2 spike glycoprotein: A computational biology approach. | LitMetric

Exploring the genomic and proteomic variations of SARS-CoV-2 spike glycoprotein: A computational biology approach.

Infect Genet Evol

Department of Genetic Engineering & Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chattogram 4331, Bangladesh; Institute of Pharmaceutical Science, School of Cancer and Pharmaceutical Sciences, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, UK.

Published: October 2020

AI Article Synopsis

  • * This study analyzed 320 whole genomes and spike protein sequences, identifying 483 unique genetic variations, including notable mutations in the spike protein that may affect its ability to interact with human cells.
  • * The findings suggest a close evolutionary link between SARS-CoV-2 and bat coronaviruses, providing insights that could aid in developing inhibitors targeted at the spike protein based on its genetic changes.

Article Abstract

The newly identified SARS-CoV-2 has now been reported from around 185 countries with more than a million confirmed human cases including more than 120,000 deaths. The genomes of SARS-COV-2 strains isolated from different parts of the world are now available and the unique features of constituent genes and proteins need to be explored to understand the biology of the virus. Spike glycoprotein is one of the major targets to be explored because of its role during the entry of coronaviruses into host cells. We analyzed 320 whole-genome sequences and 320 spike protein sequences of SARS-CoV-2 using multiple sequence alignment. In this study, 483 unique variations have been identified among the genomes of SARS-CoV-2 including 25 nonsynonymous mutations and one deletion in the spike (S) protein. Among the 26 variations detected in S, 12 variations were located at the N-terminal domain (NTD) and 6 variations at the receptor-binding domain (RBD) which might alter the interaction of S protein with the host receptor angiotensin-converting enzyme 2 (ACE2). Besides, 22 amino acid insertions were identified in the spike protein of SARS-CoV-2 in comparison with that of SARS-CoV. Phylogenetic analyses of spike protein revealed that Bat coronavirus have a close evolutionary relationship with circulating SARS-CoV-2. The genetic variation analysis data presented in this study can help a better understanding of SARS-CoV-2 pathogenesis. Based on results reported herein, potential inhibitors against S protein can be designed by considering these variations and their impact on protein structure.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7266584PMC
http://dx.doi.org/10.1016/j.meegid.2020.104389DOI Listing

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