sp. nov., isolated from spinach farming field soil.

A Gram-stain-negative bacterium, designated strain YJ09, was isolated from spinach farming field soil at Shinan in the Republic of Korea. Cells of strain YJ09 were found to be strictly aerobic, non-motile, non-spore-forming creamy-yellow rods which can grow at 20-37 °C (optimum, 30 °C), at pH 6.0-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-0.5 % (w/v) NaCl (optimum, 0 % NaCl). The 16S rRNA gene sequence analysis showed that strain YJ09 belongs to the genus with high sequence similarities to GP20-2 (98.0 %), HKS-06 (97.7 %) and JS5 (97.4 %). The results of phylogenetic analysis indicated that strain YJ09 formed a distinct phyletic line in the genus and the results of DNA-DNA relatedness studies demonstrated that strain YJ09 could be separated from its closest relatives in the genus . The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified glycolipids, an unidentified phospholipid and sphingoglycolipid. The predominant ubiquinone and polyamine components were Q-10 and spermidine, respectively. The major fatty acids were C ω7 C  and C ω7 and/or iso-C 2-OH. The DNA G+C content of this novel isolate was 65.9 mol%. On the basis of phenotypic, chemotaxonomic properties and phylogenetic analyses in this study, strain YJ09 is considered to represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is YJ09 (=KACC 19551=NBRC 113247).