AI Article Synopsis
- The study examines how diabetes affects the levels of osteopontin (OPN) and α3β1 integrin proteins during the critical process of embryo implantation in female rats.
- Results showed that OPN and α3β1 integrin levels were significantly higher in diabetic rats compared to control and treated groups, indicating potential fertility issues linked to diabetes.
- Treatment with pioglitazone and metformin improved protein levels, with pioglitazone showing greater effectiveness in restoring normal conditions.
Article Abstract
Background: Embryo implantation is a critical and multifactorial phenomenon which can be affected by any alteration in molecular micro construction of endometrium. The aim of the current study was to evaluate the effects of diabetes on osteopontin (OPN) and α3β1 integrin proteins level at the time of endometrial receptivity.
Methods: Twenty-eight female rats were divided into control, diabetic, pioglitazone-treated and metformin-treated groups. Western blot was performed to determine the OPN and α3β1 integrin proteins in rats' endometrium at the time of implantation. Data were analyzed by analysis of variance (ANOVA) and p<0.05 was considered statistically significant.
Results: OPN increased significantly in the diabetic group in comparison with control (p<0.001), metformin-treated (p=0.008) and pioglitazone-treated groups (p< 0.001). Furthermore, α3β1 integrin protein level in diabetic group had a significant difference in comparison with that of the control (p<0.001), metformin-treated (p= 0.026) and pioglitazone-treated groups (p<0.001).
Conclusion: OPN and α3β1 integrin proteins are involved in embryo implantation and their changes in diabetic condition can affect fertility. Treatment with pioglitazone and metformin improved the level of OPN and α3β1 integrin proteins while pioglitazone was more effective.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253937 | PMC |
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