https://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=pubmed&id=32496215&retmode=xml&tool=Litmetric&email=readroberts32@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09 324962152021012220231111
2059-798376Pt 62020Jun01Acta crystallographica. Section D, Structural biologyActa Crystallogr D Struct BiolCurrent approaches for automated model building into cryo-EM maps using Buccaneer with CCP-EM.531541531-54110.1107/S2059798320005513This work focuses on the use of the existing protein-model-building software Buccaneer to provide structural interpretation of electron cryo-microscopy (cryo-EM) maps. Originally developed for application to X-ray crystallography, the necessary steps to optimise the usage of Buccaneer with cryo-EM maps are shown. This approach has been applied to the data sets of 208 cryo-EM maps with resolutions of better than 4 Å. The results obtained also show an evident improvement in the sequencing step when the initial reference map and model used for crystallographic cases are replaced by a cryo-EM reference. All other necessary changes to settings in Buccaneer are implemented in the model-building pipeline from within the CCP-EM interface (as of version 1.4.0).open access.HohSoon WenSWYork Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, United Kingdom.BurnleyTomTScientific Computing Department, Science and Technology Facilities Council, Research Complex at Harwell, Didcot OX11 0FA, United Kingdom.CowtanKevinK0000-0002-0189-1437York Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, United Kingdom.engBB/P000517/1BB_Biotechnology and Biological Sciences Research CouncilUnited KingdomMR/N009614/1MRC_Medical Research CouncilUnited KingdomJournal Article20200529
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