AI Article Synopsis
- Adipose-derived stem cells (ASCs) and dedifferentiated fat (DFAT) cells are promising sources for tissue engineering, as they can be easily obtained and can differentiate into multiple cell types, but their function may decline with age.
- This study focused on comparing DFAT and ASCs from aging donors, noting that while both maintained healthy stem cell traits, DFAT cells showed higher proliferation rates and less cellular aging than ASCs.
- DNA methylation analysis revealed that aging impacted specific adipogenic genes, showing hypermethylation in three genes (PPARγ2, FABP4, LPL) and hypomethylation in one (C/EBPα), indicating a complex relationship between age and
Article Abstract
Adipose-derived stem cells (ASCs) and dedifferentiated fat (DFAT) cells are alternative cell sources in tissue engineering and regeneration because they are easily obtained and exhibit multilineage differentiation. However, aging may attenuate their regenerative potential and metabolic functions. Reports characterizing DFAT cells derived from aging donors are rare, and comparisons of DNA methylation profiles between aging ASCs and DFAT cells are poorly understood. Therefore, this study aimed to characterize DFAT cells relative to ASCs derived from aging subjects and compare the DNA methylation profiles of four adipogenic genes in these cells. ASCs and DFAT cells from aging donors exhibited characteristics similar to those of stem cells, including colony formation, proliferation, and multilineage differentiation abilities. However, compared with ASCs, DFAT cells exhibited increased proliferation, smooth muscle actin alpha (SMA-α) expression and decreased cellular senescence. DNA methylation profiling of ASCs and DFAT cells by combined bisulfite restriction analysis (COBRA) demonstrated hypermethylation patterns in three potent adipogenic genes-peroxisome proliferator-activated receptor gamma 2 (PPARγ2), fatty acid-binding protein 4 (FABP4), and lipoprotein lipase (LPL)-but hypomethylation of CCAAT/enhancer binding protein alpha (C/EBPα) in the aging group. Statistically significant differences were observed between the aging group and the young group. Epigenetic regulation maintains the stability of ASCs and DFAT cells in an age-dependent manner. Our findings suggested that although the DNA methylation patterns of three adipogenic genes correlated with hypermethylation and aging, ASCs and DFAT cells exhibited cellular stability and several stem cell characteristics, offering further opportunities for personalized regeneration and energy maintenance by adipogenesis during aging.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7505878 | PMC |
| http://dx.doi.org/10.1007/s13577-020-00379-x | DOI Listing |
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