Monospecific antisera against BCG antigen 60 were produced by all of four rabbits during immunization with precipitates containing antigen 60 cut out of gels after crossed immunoelectrophoresis. During electrolytic iodination of a crude antigen 60 preparation, preferential labeling of antigen 60 was demonstrated, and a specific radioimmunoassay was established to follow the development of anti-BCG-60 activity during immunization.
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http://dx.doi.org/10.1128/iai.16.2.662-672.1977 | DOI Listing |
J Clin Invest
January 2025
Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Metabolic reprogramming shapes tumor microenvironment (TME) and may lead to immunotherapy resistance in pancreatic ductal adenocarcinoma (PDAC). Elucidating the impact of pancreatic cancer cell metabolism in the TME is essential to therapeutic interventions. "Immune cold" PDAC is characterized by elevated lactate levels resulting from tumor cell metabolism, abundance of pro-tumor macrophages, and reduced cytotoxic T cell in the TME.
View Article and Find Full Text PDFAdv Biotechnol (Singap)
January 2025
MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, Guangdong, China.
Β-thalassemia is one of the global health burdens. The CD41-42 (-TCTT) mutation at HBB is the most prevalent pathogenic mutation of β-thalassemia in both China and Southeast Asia. Previous studies focused on repairing the HBB CD41-42 (-TCTT) mutation in β-thalassemia patient-specific induced pluripotent stem cells, which were subsequently differentiated into hematopoietic stem and progenitor cells (HSPCs) for transplantation.
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
January 2025
Laboratory of Design and Development of Innovative Knitted Textiles and Garments, Department of Industrial Design and Production Engineering, University of West Attica, 12244, Egaleo, Attica, Greece.
This study investigates the production of high-purity cellulose pulp from peach (Prunus persica) fruit wastes generated during the processing of a Greek compote and juice production industry. A three-step chemical process is used, including alkaline treatment with NaOH, organic acid (acetic and formic) treatment, and hydrogen peroxide treatment, with the goal of cellulose extraction and purification. A fractional factorial design optimized reagent levels, revealing the strong influence of NaOH concentration on α-cellulose content and degree of polymerization.
View Article and Find Full Text PDFAdv Biotechnol (Singap)
September 2024
College of Agronomy, Hunan Agricultural University, Changsha, 410128, China.
The use of nitrogen-fixing bacteria in agriculture is increasingly recognized as a sustainable method to boost crop yields, reduce chemical fertilizer use, and improve soil health. However, the microbial mechanisms by which inoculation with nitrogen-fixing bacteria enhance rice production remain unclear. In this study, rice seedlings were inoculated with the nitrogen-fixing bacterium R3 (Herbaspirillum) at the rhizosphere during the seedling stage in a pot experiment using paddy soil.
View Article and Find Full Text PDFAdv Biotechnol (Singap)
February 2024
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510006, China.
Porcine epidemic diarrhea virus (PEDV), Transmissible gastroenteritis virus (TGEV), Porcine deltacoronavirus (PDCoV) and Swine acute diarrhea syndrome coronavirus (SADS-CoV) rank among the most frequently encountered swine enteric coronaviruses (SECoVs), leading to substantial economic losses to the swine industry. The availability of a rapid and highly sensitive detection method proves beneficial for the monitoring and surveillance of SECoVs. Based on the N genes of four distinct SECoVs, a novel detection method was developed in this study by combining recombinant enzyme polymerase isothermal amplification (RPA) with clustered regularly interspaced short palindromic repeats (CRISPR)-associated proteins (Cas) 12a.
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