Methane-oxidizing bacteria (MOB) possess the metabolic potential to assimilate the highly potent greenhouse gas, CH, and can also synthesize valuable products. Depending on their distinct and fastidious metabolic pathways, MOB are mainly divided into Type I and Type II; the latter are known as producers of polyhydroxyalkanoate (PHA). Despite the metabolic potential of MOB to synthesize PHA, the ecophysiology of MOB, especially under high CH flux conditions, is yet to be understood. Therefore, in this study, a rice paddy soil receiving a high CH flux from underground was used as an inoculum to enrich MOB using fed-batch operation, then the enriched Type II MOB were characterized. The transitions in the microbial community composition and CH oxidation rates were monitored by 16S rRNA gene amplicon sequencing and degree of CH consumption. With increasing incubation time, the initially dominant Methylomonas sp., affiliated with Type I MOB, was gradually replaced with Methylocystis sp., Type II MOB, resulting in a maximum CH oxidation rate of 1.40 g-CH/g-biomass/day. The quantification of functional genes encoding methane monooxygenase, pmoA and PHA synthase, phaC, by quantitative PCR revealed concomitant increases in accordance with the Type II MOB enrichment. These increases in the functional genes underscore the significance of Type II MOB to mitigate greenhouse gas emission and produce PHA.
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http://dx.doi.org/10.1016/j.jbiosc.2020.04.006 | DOI Listing |
Mob DNA
November 2024
Herbarium and Department of Botany, Charles University, Benátská 2, CZ-12801, Prague, Czech Republic.
ACS Appl Mater Interfaces
November 2024
State Key Laboratory of Advanced Processing and Recycling of Nonferrous Metals, School of Materials Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
In response to the current policy of high storage capacity, two-dimensional (2D) materials have revealed promising prospects as high-performance electrode materials. MoB, as a type of such material, is widely regarded as an anode candidate for Li-ion batteries due to its large specific surface area and abundant ion diffusion channels; the long-term cycling stability, however, is poor owing to material pulverization during the cycle. Therefore, MoB/SiN heterojunction in this work is proposed as an anode material, with SiN acting as a skeleton, maintaining the stability of the structure, while retaining the high energy storage properties of MoB as well.
View Article and Find Full Text PDFMob DNA
October 2024
Department of Paediatrics and Adolescent Medicine, The University of Hong Kong, Hong Kong, China.
J Pediatr Endocrinol Metab
October 2024
Department of Gastroenterology, Hospital General de Zona 21, Instituto Mexicano del Seguro Social, León, México.
Objectives: Mexican children with obesity are at a higher risk of developing type 2 diabetes mellitus (T2DM). The aim of the study was to compare oral glucose tolerance test (OGTT) characteristics: time of peak glucose, glucose level ≥155 mg/dL at 1 h, presence of metabolic syndrome (MetS), sensitivity, secretion, and oral disposition index (oDI) in children with and without obesity, according to oral glucose tolerance curve shape: monophasic or biphasic.
Methods: Cross-sectional study including 143 children.
Compr Rev Food Sci Food Saf
September 2024
BioTeC+, Chemical and Biochemical Process Technology and Control, Department of Chemical Engineering, KU Leuven, Ghent, Belgium.
Flavor is a major sensory attribute affecting consumers' preference for cheese products. Differences in cheesemaking change the cheese microenvironment, thereby affecting cheese flavor profiles. A framework for tuning cheese flavor is proposed in this study, which depicts the full picture of flavor development and modulation, from manufacturing and ripening factors through the main biochemical pathways to flavor compounds and flavor notes.
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