AI Article Synopsis
- The study focuses on how the composition of sample solvents, particularly the type and amount of organic modifier, affects the solubility of tryptic glycopeptides in hydrophilic interaction liquid chromatography (HILIC).
- Findings indicate that solubility varies with the size of the attached N-glycan, showing that larger glycans lead to lower solubility, and glycopeptides with sialic acids are more likely to precipitate.
- Optimizing the sample solvent composition is essential for achieving accurate quantitative data and effective separation in HILIC.
Article Abstract
The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC). In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins. The results showed that the solubility of glycopeptides in solvents with a high acetonitrile content depends on the type of attached N-glycan. We observed lower solubility in larger glycans attached to the same peptide backbone, and we demonstrated that glycopeptides containing sialic acids precipitate more readily than those without sialic acid. Therefore, the sample solvent composition in HILIC must be carefully optimized for accurate quantitative data collection and for adequate separation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7305521 | PMC |
| http://dx.doi.org/10.1016/j.jchromb.2020.122196 | DOI Listing |
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