During nuclear maturation of most eukaryotic pre-messenger RNAs and long non-coding RNAs, introns are removed through the process of RNA splicing. Different classes of introns are excised by the U2-type or the U12-type spliceosomes, large complexes of small nuclear ribonucleoprotein particles and associated proteins. We created intronIC, a program for assigning intron class to all introns in a given genome, and used it on 24 eukaryotic genomes to create the Intron Annotation and Orthology Database (IAOD). We then used the data in the IAOD to revisit several hypotheses concerning the evolution of the two classes of spliceosomal introns, finding support for the class conversion model explaining the low abundance of U12-type introns in modern genomes.
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| http://dx.doi.org/10.1093/nar/gkaa464 | DOI Listing |
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Article Synopsis
- - The minor spliceosome is crucial for splicing U12-type introns and comprises five unique small nuclear RNAs (snRNAs), only one of which is shared with the major spliceosome.
- - Researchers used cryo-electron microscopy to create a detailed structure of the human minor spliceosome pre-B complex, including essential components like U11, U12 snRNP, and the U4atac/U6atac.U5 tri-snRNP.
- - The study reveals specific interactions between U11 snRNA and proteins, as well as unique characteristics that differentiate the minor tri-snRNP from the major tri-snRNPs during spliceosome assembly.
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