The photocatalytic fuel cell (PFC) provides an elegant approach for the construction of a light-induced self-powered sensing platform. Nevertheless, the quantification of a target with a single output signal in an ordinary PFC-based sensor is easily affected by variation of the light intensity and other environmental factors. Herein we propose a ratiometric self-powered aptasensor for highly selective detection of 17β-estradiol (E2) based on a dual-channel PFC constructed with two photoanodes which could effectively avoid the fluctuation of the light intensity. Taking the advantage of the inhibited output performance of PFC by E2 which was captured by aptamer immobilized on the photoanodes, E2 was quantified via the ratio of output power density values from dual photoanodes. Under optimum conditions, the ratiometric self-powered sensing signal was linearly related to the logarithm of E2 concentration in the range of 1 to 500 nM, with a detection limit (3S/N) of 0.12 nM. Thus, a new type of self-powered aptasensor with high accuracy and specificity was successfully developed based on PFC in cooperation with a ratiometric assay and a spatial-resolution technique.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.analchem.0c01543 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Laser-induced CdS/TiO/graphene dual photoanodes for ratiometric self-powered photoelectrochemical sensor: an innovative approach for aflatoxin B1 detection.
Mikrochim Acta
September 2024
Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou, 350108, People's Republic of China.
A ratiometric self-powered photoelectrochemical sensor based on laser direct writing technology was constructed to address the problem that the conventional single-signal detection mode was susceptible to the influence of instrumentation and environmental factors, which interfered with the detection results. Laser-induced CdS/TiO/Graphene was prepared as dual photoanodes (PA1 and PA2), which were controlled by multiplexed switches to form a photocatalytic fuel cell with Pt cathode. By modifying the aptamer of aflatoxin B1 (AFB1) on the photoanode surface, the target was specifically captured to the electrode surface to form a biological complex, which increased the steric hindrance and affected the electron transfer, thus reducing the output signal of the sensor.
View Article and Find Full Text PDFA bulit-in self-calibration ratiometric self-powered photoelectrochemical sensor for high-precision and sensitive detection of microcystin-RR.
Mikrochim Acta
June 2024
Jiangsu Key Laboratory of Materials Surface Science and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou, 213164, Jiangsu, P.R. China.
A novel high-precision aptasensor of microcystin-RR (MC-RR) is developed based on a ratiometric self-powered photoelectrochemical platform. In detail, the defective MoS/TiC nanocomposite with good photoelectric activity was designed to serve as the photoanode of the sensor for enhancing the signal and improving the detection sensitivity. In order to effectively eliminate external interferences, the key point of this ratiometric device is the introduction of the spatial-resolved technique, which includes the detection section and the reference section, generating reference signals and response signals, respectively.
View Article and Find Full Text PDFPortable self-powered electrochemical aptasensing platform for ratiometric detection of mycotoxins based on multichannel photofuel cell.
Anal Chim Acta
April 2024
Key Laboratory of Material Chemistry for Energy Conversion and Storage (Ministry of Education), Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Luoyu Road 1037, Wuhan, 430074, China. Electronic address:
Self-powered electrochemical sensors based on photofuel cells have attracted considerable research interest because their unique advantage of not requiring an external electric source, but their application in portable and multiplexed targets assay is limited by the inherent mechanism. In this work, a portable self-powered sensor constructed with multichannel photofuel cells was developed for the ratiometric detection of mycotoxins, namely ochratoxin A (OTA) and patulin (PAT). The spatially resolved CdS/BiS-modified photoanodes and a shared Prussian Blue cathode were integrated on an etched indium-tin oxide slide to fabricate the multichannel photofuel cell.
View Article and Find Full Text PDFOrthogonally Sequential Activation of Self-Powered DNAzymes Cascade for Reliable Monitoring of mRNA in Living Cells.
Adv Healthc Mater
March 2024
Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, Anhui Province Key Laboratory of Chem-Biosensing, Ministry of Education Key Laboratory of Functional Molecular Solids, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241002, China.
Ratiometric imaging of tumor-related mRNA is significant, yet spatiotemporally resolved regulation on the ratiometric signals to avoid non-specific activation in the living cells remains challenging. Herein, orthogonally sequential activation of concatenated DNAzyme circuits is, first, developed for Spatio Temporally regulated Amplified and Ratiometric (STAR) imaging of TK1 mRNA inside living cells with enhanced reliability and accuracy. By virtue of the synthesized CuO/MnO nanosheets, orthogonally regulated self-powered DNAzyme circuits are operated precisely in living cells, sequentially activating two-layered DNAzyme cleavage reactions to achieve the two ratiometric signal readouts successively for reliable monitoring of low-abundance mRNA in living cells.
View Article and Find Full Text PDFA ratiometric self-powered aptasensor for simultaneous detection of cortisol and progesterone based on spatially resolved tri-channel photofuel cell.
Biosens Bioelectron
March 2023
Key Laboratory of Material Chemistry for Energy Conversion and Storage (Ministry of Education), Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Luoyu Road 1037, Wuhan, 430074, PR China. Electronic address:
In this work, a self-powered sensor was proposed for simultaneous detection of two typical steroid hormones, namely cortisol (COR) and progesterone (P4). A tri-channel photofuel cell (PFC) consisting of three spatially resolved SnS@SnO photoanodes and one Pt cathode was designed to generate the electricity to drive the sensing process under the control of a multiplex switch. Among three photoanodes, one served as the control, while the other two were modified with COR-binding or P4-binding aptamer to respond specifically to the COR or P4 target.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!