AI Article Synopsis
- A new fluorescent aptasensor uses a DNA triangular prism to detect prostate-specific antigen (PSA), enhancing fluorescence with specific components.
- The presence of PSA leads to strong fluorescence due to DNA origami formation, while its absence results in weak fluorescence.
- The biosensor demonstrates high selectivity, a broad detection range (200 pg/mL to 300 ng/mL), and a low limit of detection (30 pg/mL), effectively identifying PSA in human serum samples.
Article Abstract
In this study, a fluorescent aptasensor is described for ultrasensitive detection of prostate-specific antigen (PSA) using DNA triangular prism as a platform for attachment of fluorophore (PicoGreen, PG), streptavidin magnetic beads (SMBs) and RecJf exonuclease as enhancers of fluorescence difference between presence and absence of target. Presence of PSA leads to the formation of the DNA origami. So, a strong fluorescence is observed following the addition of PG. while, the DNA triangular prism cannot be formed in the lack of target. Thus, a very weak fluorescence can be measured after addition of PG. The proposed biosensor indicated high selectivity, a broad linear range from 200 pg/mL to 300 ng/mL and a very low detection limit of 30 pg/mL for PSA. Applying the designed aptasensor, PSA was successfully detected in human serum samples. This work provides a new way for detection of biomarkers in clinical samples.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.aca.2020.04.071 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Spatially Preorganized Hybridization Chain Reaction for the Prompt Diagnosis of Inflammation.
Angew Chem Int Ed Engl
December 2024
State Key Laboratory of Coordination Chemistry, Department of Biomedical Engineering, College of Engineering and Applied Sciences, Chemistry and Biomedicine Innovation Center (ChemBIC), ChemBioMed Interdisciplinary Research Center, Nanjing University, Nanjing, Jiangsu, 210023, P. R. China.
Biological systems utilize precise spatial organization to facilitate and regulate information transmission within signaling networks. Inspired by this, artificial scaffolds that enable delicate spatial arrangements are desirable to increase the local concentration of reactants, expedite specific interactions, and minimize undesired interference. In this study, we presented an integrated biosensing nanodevice, termed TRI-HCR, in which hybridization chain reaction (HCR) probes were precisely organized on a triangular DNA origami nanostructure (TRI) with finely-tuned distance, quantity, and pattern.
View Article and Find Full Text PDFComprehensive mapping of molecular cytogenetic markers in pitaya () and related species.
Front Plant Sci
December 2024
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro-bioengineering, College of Life Science, Guizhou University, Guiyang, Guizhou, China.
Pitaya (; 2n=22) is an important fruit crop from the family, originally domesticated in Mexico and the USA, and is now widely cultivated for its nutritional benefits. It is characterized by its distinctive triangular-shaped stems and large, showy flowers, thriving in arid and semi-arid environments, particularly in hot, dry climates. However, systematic chromosomal studies, including chromosomal mapping of cytogenetic markers in pitaya, are limited, presenting challenges for its cytogenetic improvement.
View Article and Find Full Text PDFEnhanced liquid-liquid phase separation of stress granules in a reconstructed model and their cytoplasmic targeting using a DNA nanodevice.
J Mater Chem B
December 2024
Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China.
Biomolecular condensates (BCs) are crucial membraneless organelles formed through the process of liquid-liquid phase separation (LLPS) involving proteins and nucleic acids. These LLPS processes are tightly linked with essential cellular activities. Stress granules (SGs), functioning as cytoplasmic BCs, play indispensable roles in maintaining cellular homeostasis and are implicated in diseases like cancers and neurodegenerative disorders.
View Article and Find Full Text PDFA New Cryptic Species of Splitfin Fish from Taiwan with Revision of the Genus (Acropomatiformes: Synagropidae.
Zool Stud
July 2024
Biodiversity Research Center, Academia Sinica, Taipei 11529, Taiwan. E-mail: (Lin).
A new species of splitfin fish, sp. nov., from Dongsha Island, Taiwan, South China Sea, is described based on its morphological characteristics, including osteology and otolith morphology, and DNA sequence.
View Article and Find Full Text PDFA versatile approach for geometry-based self-assembly of DNA-protein hybrid nanostructures using histone-DNA interactions.
Chem Commun (Camb)
January 2025
King Abdullah University of Science and Technology, Biological and Environmental Science and Engineering Division, Thuwal 23955-6900, Saudi Arabia.
We report an enhanced versatility in constructing DNA-protein hybrid nanostructures using histone-DNA complexes (HDs). By leveraging HDs, we demonstrate precise and scalable assembly of DNA origami tiles and a 2D triangular nanostructure. Our results extend the potential applications of DNA nanotechnology from the nanoscale to the microscale without the need for complex pre-designs.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!