AI Article Synopsis
- Researchers successfully isolated the flavin semiquinone intermediate from flavoproteins, like cryptochromes, using sodium ascorbate to reduce the FMN cofactor in water.
- The semiquinone was created in a specially designed hydrophobic environment made from modified polyethyleneimine.
- The intermediate was characterized through various techniques, including UV-Visible, fluorescence, and electron paramagnetic resonance (EPR) spectroscopies.
Article Abstract
The elusive flavin semiquinone intermediate found in flavoproteins such as cryptochromes has been obtained in aqueous solution by single electron reduction of the natural FMN cofactor using sodium ascorbate. This species was formed in the local hydrophobic microenvironment of a modified polyethyleneimine and characterized by UV-Visible, fluorescence and EPR spectroscopies.
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| http://dx.doi.org/10.1039/d0ob00864h | DOI Listing |
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