Non-enzymatically isolated primary dermal progenitor fibroblasts derived from fetal organ donations are ideal cell types for allogenic musculoskeletal regenerative therapeutic applications. These cell types are differentiated, highly proliferative in standard in vitro culture conditions and extremely stable throughout their defined lifespans. Technical simplicity, robustness of bioprocessing and relatively small therapeutic dose requirements enable pragmatic and efficient production of clinical progenitor fibroblast lots under cGMP standards. Herein we describe optimized and standardized monolayer culture expansion protocols using dermal progenitor fibroblasts isolated under a Fetal Transplantation Program for the establishment of GMP tiered Master, Working and End of Production cryopreserved Cell Banks. Safety, stability and quality parameters are assessed through stringent testing of progeny biological materials, in view of clinical application to human patients suffering from diverse cutaneous chronic and acute affections. These methods and approaches, coupled to adequate cell source optimization, enable the obtention of a virtually limitless source of highly consistent and safe biological therapeutic material to be used for innovative regenerative medicine applications.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/7651_2020_295 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Alteration of skin fibroblast steady state contributes to healing outcomes.
bioRxiv
December 2024
Department of Pathology, Yale University, New Haven, CT 06520, USA.
Fibroblasts display complex functions associated with distinct gene expression profiles that influence matrix production and cell communications and the autonomy of tissue development and repair. Thrombospondin-2 (TSP-2), produced by fibroblasts, is a potent angiogenesis inhibitor and negatively associated with tissue repair. Single-cell (sc) sequencing analysis on WT and TSP2KO skin fibroblasts demonstrate distinct cell heterogeneity.
View Article and Find Full Text PDFAdipose Tissue, Regeneration, and Skin Health: The Next Regenerative Frontier.
Aesthet Surg J Open Forum
November 2024
Adipose tissue, or fat compartments, has long been considered a storage depot and an energy source. However, a large part of new research, starting with the discovery of adipose-derived stem cells, has redirected this thinking toward the tremendous regenerative capacity that adipose tissue possesses when it is healthy. This has resulted in multiple technologies being explored with fat as a basis or with fat as a target aiming at the stimulation of new small hyperplastic adipose cells exuding adipokines and encouraging the proliferation of a whole host of progenitor cells that can have positive effects on many organ systems.
View Article and Find Full Text PDFEngineered biomimetic nanovesicles-laden multifunctional hydrogel enhances targeted therapy of diabetic wound.
Mater Today Bio
December 2024
Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong, University of Science and Technology, Wuhan, 430022, China.
Article Synopsis
- Angiogenesis is crucial for healing diabetic wounds, and endothelial progenitor cell-derived extracellular vesicles (EPC-EVs) can enhance this process, but their use is limited by low yield and targeting issues.
- The study developed biomimetic nanovesicles (EPC-NV) from EPCs and modified them with cRGD peptides (mEPC-NV) for better targeting of endothelial cells.
- A dual hydrogel network was created that combined an acellular dermal matrix with light-cured gelatin to sustainably release mEPC-NV while providing antioxidant and antibacterial properties, making it a promising approach for diabetic wound treatment.
Graft-versus-host disease variety toxic epidermal necrolysis. Case report.
Bol Med Hosp Infant Mex
November 2024
Servicio de Trasplante de células progenitoras hematopoyéticas, Hospital Infantil de México Federico Gómez. Mexico City, Mexico.
Article Synopsis
- An 11-year-old girl developed severe Grade IV skin graft-versus-host disease resembling toxic epidermal necrolysis after receiving hematopoietic progenitor cell transplantation from a fully compatible unrelated donor.
- On the 35th day post-transplant, she exhibited rash-like lesions, and a skin biopsy confirmed a diagnosis of TEN-like acute cutaneous GVHD, highlighting serious skin damage and inflammation.
- After adjusting her immunosuppressant medications, the patient achieved an 80% reduction in symptoms, indicating a positive response to treatment.
PF-PEG@ASIV-EXO Hydrogel Accelerates Diabetic Wound Healing by Ferroptosis Resistance and Promoting Angiogenesis.
ACS Biomater Sci Eng
October 2024
College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China.
Article Synopsis
- Astragaloside IV (ASIV) enhances the growth of endothelial progenitor cells (EPCs), which are crucial for wound healing, while a new composite delivery system (PF-PEG@ASIV-EXO) utilizes exosomes and hydrogels to target these cells effectively.
- The study evaluated the biocompatibility of the PF-PEG@ASIV-EXO system and its effects on EPC function, including cell viability, blood vessel formation (angiogenesis), and cellular health in high-glucose environments resembling diabetes.
- Results from diabetic rat wounds showed that PF-PEG@ASIV-EXO not only increased the healing process but also elevated important markers for blood vessels and reduced harmful cell death,
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!