Background: Dengue fever is currently endemic in tropical and subtropical countries worldwide and effective drug against DENV infection is still unavailable. Porcupine dates, which are traditionally used to treat dengue fever, might contain potential anti-dengue compounds. Two porcupine dates, black date (BD) and powdery date (PD) from Himalayan porcupine (), were investigated for their antiviral activities against DENV-2 in vitro.
Methods: The methanol crude extracts (MBD and MPD) were prepared from the raw material of porcupine dates. The tannin-rich fractions (BDTF and PDTF) were isolated from their methanol crude extracts using column chromatography. The presence of tannins in BDTF and PDTF extracts was determined by fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) analyses. The cytotoxicity and anti-DENV-2 activities including virus yield inhibition, virucidal, virus attachment and pre-treatment assays of the extracts were examined in Vero cells.
Results: Our findings revealed that all the extracts of porcupine dates exhibited antiviral activity against DENV-2 in Vero cells. The IC of BDTF and PDTF were 25 µg/mL and 11 µg/mL respectively, while their methanol crude extracts demonstrated lower antiviral efficacy (IC ≈ 101-107 µg/mL). BDTF and PDTF also exerted a similar higher virucidal effect (IC of 11 µg/mL) than methanol crude extracts (IC ≈ 52-66 µg/mL). Furthermore, all the extracts inhibited the attachment of DENV-2 by at least 80%. Pre-treatments of cells with BDTF and PDTF markedly prevented DENV-2 infection when compared to methanol crude extracts.
Conclusion: This study suggests that porcupine dates possess antiviral properties against DENV-2, which is attributed to its tannin compounds.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238553 | PMC |
| http://dx.doi.org/10.1186/s13020-020-00329-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An extinct north American porcupine with a South American tail.
Curr Biol
June 2024
Florida Museum of Natural History, Dickinson Hall, University of Florida, Gainesville, FL 36211, USA.
New World porcupines (Erethizontinae) originated in South America and dispersed into North America as part of the Great American Biotic Interchange (GABI) 3-4 million years ago. Extant prehensile-tailed porcupines (Coendou) today live in tropical forests of Central and South America. In contrast, North American porcupines (Erethizon dorsatum) are thought to be ecologically adapted to higher-latitude temperate forests, with a larger body, shorter tail, and diet that includes bark.
View Article and Find Full Text PDFThe Systematic Position of a Large Porcupine (Hystrix, Hystricidae, Rodentia) from the Pleistocene of Vietnam (Lang Trang Cave).
Dokl Biol Sci
November 2020
Borissiak Paleontological Institute, Russian Academy of Sciences, 117647, Moscow, Russia.
The dental remains of the porcupine from the Pleistocene deposits of the Lang Trang cave in northern Vietnam are described. Previously, this porcupine was identified as Hystrix brachyura Linnaeus, 1758 or H. indica Kerr, 1792.
View Article and Find Full Text PDFAnti-dengue virus serotype 2 activity of tannins from porcupine dates.
Chin Med
May 2020
1Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor Darul Ehsan Malaysia.
Background: Dengue fever is currently endemic in tropical and subtropical countries worldwide and effective drug against DENV infection is still unavailable. Porcupine dates, which are traditionally used to treat dengue fever, might contain potential anti-dengue compounds. Two porcupine dates, black date (BD) and powdery date (PD) from Himalayan porcupine (), were investigated for their antiviral activities against DENV-2 in vitro.
View Article and Find Full Text PDFPorcupine gnaw marks on a Late Pliocene bone from the Upper Siwaliks exposed near Village Khetpurali (Haryana, India).
An Acad Bras Cienc
February 2019
Department of Anthropology, Panjab University, Sector 14, 160 014 Chandigarh, India.
Bone accumulation by porcupines at archaeological sites is well known. However, in paleontological sites such a taphonomical occurrence is rather rare. We here report porcupine (Hystrix sp.
View Article and Find Full Text PDFAntioxidant and Intracellular Reactive Oxygen Species/Reactive Nitrogen Species Scavenging Activities of Three Porcupine Bezoars from .
Pharmacognosy Res
January 2017
School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor, Malaysia.
Background: Porcupine dates are phytobezoar stones that are used in Traditional Chinese Medicine (TCM) treatments against cancer, postsurgical recovery, dengue fever, etc. The medicinal values have not been scientifically investigated due to the availability and high pricing of the dates.
Objectives: This paper represents the first report on the phytochemical content, antioxidant and intracellular reactive oxygen species (ROS)/reactive nitrogen species (RNS) scavenging properties of the extracts of three porcupine dates: grassy date (GD), black date (BD), and powdery date (PD).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!