AI Article Synopsis
- A method was developed to measure T-2 and HT-2 toxins in feed and food using immunoaffinity columns and triple quadrupole LC-MS/MS for analysis.
- The method was validated according to EU regulations and ISO/IEC 17025 standards and has a detection range of 5.0-750 µg/kg.
- It was used to test 105 feed samples and 17 food samples in Southern Italy from 2015-2019, finding T-2 toxins in 30.5% of feed and 11.8% of food, and HT-2 toxins in 61.9% of feed and 17.6% of food.
Article Abstract
A method to determine T-2 and HT-2 toxins, both in feed and food, is described. The method is based on sample clean-up by immunoaffinity columns, followed by triple quadrupole LC-MS/MS determination. It was in-house validated in compliance with the European Union legislation, in the concentration range 5.0-750 µg kg and accredited according to the ISO/IEC 17025 standard, for the scope of official control of feed and food. The method was applied to monitor the contamination levels of T-2 and HT-2 toxins in 105 feed samples, both raw materials and compound feed and 17 cereals and derived products for human consumption, collected in Southern Italy between 2015 and 2019. T-2 toxin was quantified in 32 feed (30.5%) and in 2 food samples (11.8%). HT-2 toxin was quantified in 65 feed (61.9%) and in 3 food (17.6%) samples.
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| http://dx.doi.org/10.1080/19393210.2020.1771776 | DOI Listing |
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