A "turn-on" fluorescence sensor was developed for total detection of fumonisin B, B and B (FB, FB and FB) in maize samples. Rhodamine B isothiocyanate (RBITC) and gold nanoparticles (AuNPs) were employed as the energy donor-acceptor pairs. The intensity of restored fluorescence was inversely proportional to the concentration of the FBs. The limit of detection (LOD) of the sensor for FB was 23.80 pg/mL. The coefficient of variation (CV) was both less than 7% for intra- and inter-assay. The linear range was from 51.39 to 2125.92 pg/mL. The average recoveries of FBs from maize samples were ranged from 88.7% to 107.2%. The correlation coefficient of the results between the developed sensor and LC-MS/MS was 0.9970. To fulfill the procedure of the assay, only 46 min was needed. These results suggest that the fluorescence "turn-on" sensor has great potential applications in the analysis of FBs in maize samples.
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